Accordingly, the following questions must be answered in the context where the tension between newspapers and new media departments seems persistent: What will be the destiny of metropolis newspapers if they deviate from converging news production? Will other types of newspapers, due to their loss of market competitive edge to new media outlets, shift to the role of ‘Party Mouthpiece’ that has previously been performed by the party organs? Evidently, these questions can be explored in future research.
Target de zhíyè shengyá yú 2006 nián 5 yuè jiéshù, dangshí yi míng lumang de tóngshì yòng gòuwù che fanfù pengjíle lián zài tui shàng. Ta yú liù yuèfèn zài xi lì yà dàxué bìyè dianli shàng danrèn lúnyi. Zài ben yuè wan xie shíhòu, women kaiche dào mi'er fú dé. Tongguò zhijiage huí dào míng ní abo lì si de huíchéng, yi liàng zhijiage jingchá xiaoduì pao dàole I-94 zhou jì gonglù de che shàng. Che zonggòng liánlián zàicì shòushang. Ta xùnsù dàole zhongguó.
67 The side with higher audience costs is less likely to back down in a foreign crisis and therefore able to signal its intentions to other states more credibly than states with lower audience costs. Fearon, J.D., “Domestic political audiences and the escalation of international disputes,” American Political Science Review, Vol. 88, No. 3 (1994), pp. 577–92. Weiss, “Powerful patriots: nationalism, diplomacy and the strategic logic of anti-foreign protest.”

Wúlùn shì zhongzú háishì qíta yuányin, wo de fùmu dou bù zànchéng wo yu xila de hunyin. Wo de muqin anpái wo de dìdì an dí hé 1960 nián bìyè de ai kè sai tè zhunbèi xuéxiào de yiqún xiàoyou yiqi qù zhongguó luxíng. ai kè sai tè bìyè sheng de luxíng tuán dangshí shì meiguó zhù huá dàshi de tian'anmén guangchang dà túsha. Wo bèi yaoqiú péibàn an dí, yi quèbao ta fúyòng ta di yàowù. Zhè cì luxíng yú 1996 nián 4 yuèdi kaishi, chíxùle san gè xingqí. Dang wo huí dào míng ní abo lì si shí, xila hé háizimen yiqi zoule, hái you dà píngmù diànshì hé qíta de cáiwù. Dang wo da diànhuà gei shànghai, méiyou rén huídá de shíhòu, wo you yigè zhèyàng de qíngkuàng.
Chongman yíwèn, wo xiàng fóluólidá zhou Clearwater zhensuo de zhuguan fasòngle yi feng diànzi yóujiàn, gai wangzhàn de Sheila céngjing xianshìguò wo de wangzhàn. Ta huídá shuo, zhensuo yu xila méiyou rènhé yèwù guanxì. Xianrán wo bèi piàn liao dàng wo miàn duì xila shí, ta shuo jìhuà you yigè zuìhòu yikè de biànhuà. Qíshí zhège chéngxù shì zài luo lì bei kaluóláinà dàxué yiyuàn wánchéng de. Rán'ér, zhè jia yiyuàn de wangzhàn méiyou liè chu shuluanguan jiéza de nìzhuan shì qí tígong de yiliáo fúwù zhi yi. Zài ji zhou zhihòu, yu xila de fènnù de diànzi yóujiàn jiaoliú zhihòu, wo xiangxìn xila yijing qi piànle wo.
To provide a quantitative measure of (GGGGCC)n hexanucleotide expansion in C9ORF72, 100 ng of genomic DNA was amplified by touchdown PCR using primers shown in Supplemental Table 2, in a 28-μL PCR reaction consisting of 0.2 mM each of 7-deaza-2-deoxyguanine triphosphate (deaza-dGTP) (NEB), dATP, dCTP, and dTTP, 7% DMSO, 1× Q-Solution, 1× Taq PCR buffer (Roche), 0.9 mM MgCl2, 0.7 μM reverse primer (4 GGGGCC repeats with an anchor tail), 1.4 μM 6FAM fluorescently labeled forward primer, and 1.4 μM anchor primer corresponding to the anchor tail of reverse primer (Supplemental Table 2). During the PCR, the annealing temperature was gradually decreased from 70°C and 56°C in 2°C increments with a 3-minute extension time for each cycle. The PCR products were purified by QiaQuick PCR purification kit (Qiagen) and analyzed using an ABI3730 DNA Analyzer and Peak Scanner Software v1.0 (Life Technologies).

Wénlín 4.3 bǎn hái wánquán zhīchí Adobe de Han Sans zìtǐ, zhè shì yī zhǒng xīn de Pan-CJK zìtǐ xì (kě miǎnfèi xiàzài), hái gǎijìnle zìtǐ xuǎnzé gōngnéng de yì yòng xìng. Qítā xīn gōngnéng hé shēngjí de gōngnéng bāokuò︰yāsuō MP3 géshi lùyīn; nèi zhì wǎngluò sōusuǒ; xīn de fāyīn, dǎoháng hé lièbiǎo děng zēngqiáng gōngnéng. Wénlín 4.3 bǎn wánquán zhīchí Unicode8 CJK de kuòzhǎn IE zhīchí, ràng Wénlín zhuānshǔ CDL zìtǐ yǐnqíng de wénzì miáoshù zǒngshù dádào 103, 510. Huānyíng fǎngwèn www.wenlinshangdian.com gòumǎi Wénlín 4.3 bǎn, shòujià wéi$99. Fúhé zīgé de yònghù kě xiǎngshòu shēngjí jiàgé. Shēngjí nèiróng de wánzhěng lièbiǎo qǐng cānjiàn: http://tinyurl.com/wen43.

Guanyú zìwo yìshí de zhutí, shouxian rènshi dào ta shì jiézòu de dírén. Ta shì yi zhong fushí xìng de yingxiang, daozhì jiézòu shiqù. Jiézòu de biaoxiàn xuyào mou zhong zhùyì lì, jízhong zài sixiang de nèibù yàosù yuqí zhíxíng ér bùshì zhèngzài zhíxíng de lì chéng. Zhùyì rènhé shìqíng shì yi zhong fenxin, daozhì jízhong shiqù. Biaoyan zhe bùnéng zhùyì zìji huò ta de yùndòng, bìng qiwàng biaoxiàn liánghao. Ta bìxu shiqù biaoyan benshen.

Wo juédìng duì míngnísudá zhou shàngsù fayuàn tíchu shàngsù. Wo ye pìnqingle yi wèi xin de l?shi tángnàdé·xi er si tè lún (Donald Hillstrom) lái dàibiao wo. Women zuò chule yigè wúchi de huyù, daozhì fatíng tuifanle fà xi qí de juédìng. Ba zhège àn zi huán geile shenpàn faguan, zhishì ta bùdé bù xuanbù wo qizi céngjing maiguò fángzi de qián shì yi dàikuan háishì liwù de xíngshì chuxiàn.

Zài 1987 nián niándi de mou gè shíhòu, an gàosù wo ta huáiyùnle. Ta xiesidili de yaoqiú wo jià gei ta. Wo hen danxin yu qíngxù bù wendìng de nurén xiànrù zhongshen de guanxì. Lìng yigè wèntí shì an zhèngzài jieshòu li zhìliáo yìyù zhèng. Yi zhi li yinqi zhìlì chídùn. Yisheng zài chufang lidiàn zhiqián yinggai wèn'an, ta shìfou you xìng huódòng, dànshì tamen hushìle zhè yidian. Wo you yigè yìnxiàng shì tamen duncù an duòtai. Jíshi bu shì yisheng, ta dí gege kendìng shì. Méiyou zixún wo, ta anpáile zài dàxué yiyuàn zhongzhi tai'ér. Zài shoushù wánchéng hòu, wo zài yiyuàn kànguò an.


The last two sections show some potential changes in the dominant path of media convergence adopted by the local press industry. Existing studies indicate that institutional and organisational factors considerably influence the journalists’ perception of their professional roles (Tao and Zhang, 2014; Wu et al., 1996; Zhang and Wu, 2016). Long-term attention is equally worthwhile with regard to whether the future structural adjustment of the local press industry in the area of media convergence will change the journalists’ identification of their roles.
This decision means that the Chinese media must be dedicated to Party and government propaganda whilst earning profits and seeking for industry development. This institutional framework enabled the metropolis newspapers to emphasise the function of profitability that emerged in the mid-1990s. Differing from the party organs, the metropolis newspapers have consciously catered to the preferences of the audience in terms of content, including considerable emphasis on the timeliness, relevance and interesting aspects of the news, concentration on social and personal stories and a continuous increase in the proportion of entertainment and leisure contents. Zhao (1998: 159) opined that these measures are more about supplement than disobedience to the party organs, thereby extending the official ideology further to the social, personal and even mental domains.

“Cong guigen qingjie dao bentuhua: yi Xin Ma Panyu huiguan wei gean yanjiu, kan haiwai huaren shetuan yu Guangdong qiaoxiang guanxi de yanbian.” 从归根情结到本土化:以新马番禺为个案研究,看海外华人社团与广东侨乡关系的演变  (From Homecoming to China to Localisation: Case Studies of Singapore and Malaysian Panyu Associations) presented at ‘The International Conference on “The Development Trends of the Guangdong Overseas Chinese Organizations’ in Guangzhou, organized by Guangdong Returned Overseas Chinese Association and Guangdong Society of Overseas Chinese Studies, 9 December 2001.
(A) Production of Hb9::RFP+ iMNs and survival tracking by time-lapse microscopy. (B and C) Survival of control (CTRL) and C9ORF72 ALS patient (C9-ALS) iMNs with a 12-hour pulse treatment of excess glutamate shown for each individual line separately (B), or for iMNs from all lines in aggregate (C). For B and C, n = 90 iMNs per line for 3 control and 2 C9-ALS lines, iMNs quantified from 3 biologically independent iMN conversions per line. (D) Survival of control and C9ORF72 ALS patient iMNs after withdrawal of neurotrophic factor supplementation. iMNs from all control or C9ORF72 patient lines shown in aggregate. n = 90 iMNs per line for 3 control and 3 C9-ALS lines, iMNs quantified from 3 biologically independent iMN conversions per line. (E and F) Survival of control and sporadic ALS (sALS) patient lines after glutamate treatment (E) or withdrawal of neurotrophic factor supplementation (F). iMNs from all control or C9ORF72 patient lines shown in aggregate. n = 90 iMNs per line for 3 control and 6 (E) or 5 (F) sporadic ALS lines, except sALS6 which had 60 (E) or 40 (F) iMNs counted. iMNs quantified from 3 biologically independent iMN conversions per line. (G–I) Immunofluorescence analysis of total TDP-43 (G) and quantification of the ratio of nuclear to cytoplasmic TDP-43 in control, C9-ALS (H), or sporadic ALS (I) iMNs. Ratio of nuclear to cytoplasmic TDP-43 in individual iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 6 days. iMNs from 2 controls and 2 C9-ALS patients (H) or 4 sporadic ALS patients (I) were quantified. n = 30 (controls), 30 (C9-ALS), or 36 (sporadic) iMNs per line per condition from 2 biologically independent iMN conversions of 2 control, 2 C9-ALS, or 4 sporadic ALS lines were quantified. Each gray circle represents a single iMN. Median ± interquartile range. Unpaired Mann-Whitney test. Scale bars: 5 μm. Dotted lines outline the nucleus and cell body. For iMN survival experiments, significance was measure by 2-sided log-rank test using the entire survival time course. The day of differentiation stated on each panel indicates the day of differentiation on which the experimental treatment or time course was initiated.
Zài 1857 nián, màikè kuí si dùn ba zìji duì gongsi de xìngqù mài geile ta de zhízi. Duolúnduo de Massey Harris gongsi shougòule L.D Sawyer gongsi 40%de gufèn, hébìng hòu de gongsi chéngwéi Sawyer Massey gongsi. Ta chíxù dào 1910 nián. Ránhòu,Massey Harris gongsi zìji yùnzuò, zhídào ta yu Ferguson gongsi zài 1953 nián hébìng chéngwéi Massey-Ferguson.
Zhèxie qiánghuà shèbèi you zhù yú jiànlì kouqiang wénhuà. Zhè zhong wénhuà duìyú yíshì xìng biaoxiàn de zhongshí dù you hen gao de yaoqiú. Bìxu jì zhù yiqiè de rén fà zhan chu baoshou de xintài, bù yuàn jieshòu xuyào bù xuéxí hé chóngxin xuéxí mou xie shìwù de biànhuà. Kàn qilái yigè zérèn, bìxu jì zhù suoyou zhèxie zhishì, ér bù qiúzhù yú xiezuò. Lìng yi fangmiàn, zhishì biàn de gèngjia gèrén huà, bìngqie zài jìyì zhong baoliú ér bùshì zài zhi shàng biaodá shí geng mìqiè. Zài yuánshi wénhuà yi zongjiào wéi zhongxin de chéngdù shàng, mei zhong yíshì huò jìyì xíngwéi dou chéngwéi yi zhong jiang rén yu zuxian de líng hé shén liánxì qilái de daogào. Zài zhèyàng de wénhuà zhong youyigè shèqu jingshén, shìzì shèhuì quefá. Zhè zhong lèixíng de jìyì zài jingshén shàng bi yóu shují zuchéng de wénhuà lèixíng gèng qiángdà hé gèng fengfù, suirán ta ye kenéng shì yi zhong xiàol? jiào di de baocún zhishì de fangshì.
Yu yiqián jièshào de jingyàn yiyàng, wo de cháxún cóng wèi daozhìguò rìqí. Ji gè nurén yòu rén de tíyì zuò mou xie shìqíng, dàn wo yongyuan bùnéng ba tamen fàng zài yigè shíjian hé dìdian jiànmiàn. Bùxiang fachu gè rén diànhuà hàoma huò diànzi yóujiàn dìzhi, women zhèngzài tongguò women de dàima míngcheng zài wangzhàn nèi jìnxíng tongxìn. Zuìzhong, women bìxu xianghù xìnrèn xiangguan de xìnxi, zúyi anpái zài ròuti shàng ju háng huìyì. Nà zhong qíngkuàng cónglái méiyouguò.
Wo juédìng kaiche huí dào Cottage Grove zhensuo de shouzhong, xúnwèn shìfou shì zhenshí de. Guìtái dì nà wèi nushì zài hòutái zixúnle bàn gè xiaoshí de shíjian, ránhòu gàosù wo, youguan yinsi quán de guidìng jiang bù huì ràng Allina huídá wo de wèntí. Wo xuyào bìngrén zìji xiàng wo tòulù zhèxie xìnxi. Wo yaoqiú hé zhensuo zhurèn shuohuà. Suirán ta gàosù wo tóngyàng de shìqíng, dàn ta geile wo yi fèn zhensuo de wénjù, xianshì biaozhì hé dìzhi zài yòubian. Zài xila de “xìn” zhong, zhèxie dou zài zuobian. Zhè jiùshì ta huì shuo de. Rúguo xiwàng tóngyì pilù wo yaoqiú de ziliào, wo ye huì shou dào xila de tóngyì shu, tiánxie bìng qianzì.
Bùguò, huí dào meiguó, wo zài 2002 nián 9 yuè 8 rì shou dàole yi feng diànzi yóujiàn, nèiróng rúxià:“Wo hen yíhàn, zhidào ni de qizi shàng cì méiyou huáiyùn... Wo zài ci tígong yixie xìnxi kaol?. Shouxian, ni hé ni qizi de niánlíng dou bù xiao, huáiyùn de kenéng xìng huì hen di, dàyue shì 10%, ér ying'ér jixíng l? ye huì hen gao. Qícì, ni de jingzi de zhìliàng hé liúdòng xìng bùshì nàme hao, ni qizi de zigong nèi mó ye bùgòu hao... Dì san, ni qizi de jiànkang bù hao, you tángniàobìng hé gao xieya, huáiyùn huì èhuà zhèxie jíbìng. Shùnyì dì,IVF de fèiyòng chaoguò 20,000 yuán, chéngxù fùzá. Xiwàng yishàng xìnxi duì nín de juédìng shì youyòng de.“
Wo yiwéi jiéhun jiudiàn jingli de yìsi shì shuo, rúguo women bù tóngyì, wo de qizi huì shì wo keyi tuifan de rén. Shìshí zhèngmíng bùshì zhen de. Xiàng wo de dì yi rèn qizi, lián jingcháng ting qilái xiàng yigè pò jìlù de jìlù: Wo méiyou luxíng wo zhàngfu de zhízé. Wo cónglái méiyou gei ta “qizi de lìchang”. Wo shì zìsi de wo zhi xiang zìji qù xiangshòu. Dang wo duì wo de shengyin biaoxiàn chu rènhé fánnao shí, wo duì ta hen shengqì. Ta shengqìle you yicì, zài ting liánlián dehuà de shíhòu, wo juédìng bù qù zhenglùn, zhishì baochí chénmò. Wo shíwu fenzhong zuoyòu jiù gen wo shuole yijù huà. Wo gandào jingyà de shì, dang zhège sùsòng jiéshù de shíhòu, liánxì rén shuo zhè shì wo tingguò ta de ji cì zhi yi.
Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease with diverse etiologies. Therefore, the identification of common disease mechanisms and therapeutics targeting these mechanisms could dramatically improve clinical outcomes. To this end, we developed induced motor neuron (iMN) models from C9ORF72 and sporadic ALS patients to identify targets that are effective against these types of cases, which together comprise approximately 90% of patients. We find that iMNs from C9ORF72 and several sporadic ALS patients share 2 common defects — impaired autophagosome formation and the aberrant accumulation of glutamate receptors. Moreover, we show that an anticoagulation-deficient form of activated protein C, 3K3A-APC, rescues these defects in both C9ORF72 and sporadic ALS iMNs. As a result, 3K3A-APC treatment lowers C9ORF72 dipeptide-repeat protein (DPR) levels, restores nuclear TDP-43 localization, and rescues the survival of both C9ORF72 and sporadic ALS iMNs. Importantly, 3K3A-APC also lowers glutamate receptor levels and rescues proteostasis in vivo in C9ORF72 gain- and loss-of-function mouse models. Thus, motor neurons from C9ORF72 and at least a subset of sporadic ALS patients share early defects in autophagosome formation and glutamate receptor homeostasis and a single therapeutic approach may be efficacious against these disease processes.

5 Yuè 25 rì zuoyòu, míng ní abo lì si shì zhang hòuxuan rén bào bó·ka ní (Bob Carney) da diànhuà xúnwèn wo shìfou huì hé ta yiqi churèn míngnísudá zhou fù zhouzhang. Ta zhèngzài gònghédang xiaoxuésheng zhong danrèn “wenhé, jìnqu de gònghédang rén”. Bào bó xiàng wo baozhèng, wo bùbì zuò rènhé gongzuò. Shenqing fèi shì $ 300, dàn ta huì wèi wo zhifù. Wo tíchu zhifù 200 meiyuán de fèiyòng. Zhè shì wo keyi chuli de shìqíng - ràng biérén dàitóu canyù zhèngzhì yùndòng. Zài 2010 nián 9 yuè 10 rì juxíng de 2010 nián gònghédang zhuxí xiaozu zhong, bào bó hé wo pái zài sì míng hòuxuan rén zhong de dì èr míng. Yu tangmu·ài mò màn de 107,600 piào xiang bi, women yongyou yue 9,800 piào huò 7.5%De piàoshù.


Wénlín yì wéi “qúnyīng huì” yǔ gōngsī míng xiāngtóng de shì, Wénlín Yánjiūsuǒ de chéngxùyuán dàitóurén dōu shì shēnjiān Hànyǔ yǔyánxuéjiā hé jìsuànjī kēxué gōngchéngshī liǎng zhǒng shēnfèn. Guānchá děngshì liǎngbiān, zhè ràng Wénlín Yánjiūsuǒ de chuàngshǐrén hé zǒngcái Tuōmǎsī E. Bìxiǎopǔ (Thomas E. Bishop) chóngxīn fāxiàn le Hànyǔ shénmì de 36 bǐ “zìmǔ”, jìn'ér yīláoyǒngyì de jiějué le Unicode “quēzì” de wèntí. Zhèxiē chuántǒng jiǎoběn yuánsù yīzhí yǐlái dōu bèi ruǎnjiàn kāifāzhě suǒ hūshì. “CDL yīzhí shì Wénlín de yī bùfèn, dàn wǒmen wèi fāxiàn qiánzài yǔyán, zhídào Wénlín 4.0 bǎn tuīchū. Rújīn yònghù kěyǐ chákàn bìng cāokòng rènhé kě zài bǐhuà kuàng zhōng chákàn de zìfú de CDL miáoshù,” Bìxiǎopǔ shuōdào. Wénlín Yánjiūsuǒ shì Unicode Xiéhuì de róngyù huìyuán, zì 2000 nián yǐlái yīzhí zhìlìyú míhé CJK shùjù chǔlǐ shang de chājù.
*  “Yuanyuan, changyu, xitong: Xinhua wenxueshi de jiegouxing xiezuo” 渊源、场域、系统:新华文学史的结构性写作 (Origins, Sites, Systems: Structural Writings of Singapore Chinese Literary History), presented at the 2nd Asia-Pacific Chinese Literature International Conference on “Writings of Chinese Literary History,” in Taipei, organized by the Department of Chinese Literature, National Taipei University, 5 October 2012.
Zhège nurén, xila, shì yigè xidú zhe, bù shì zuhù, ér shì dang yin junzi zàichang shí jingcháng guanggù jími gongyù de rén. Ta hái you ji gè háizi. Zuìxiao de ai li ka, yigè yuè qián jiù chushengle. Xila hé wo hen kuài chéngwéi xìng bànlu. Yinwèi shuìjiào de háizi zhèngzài zhànjù suoyou de chuángwèi, suoyi women shouxian zài míng ní abo lì si beibù de zufáng li zhànle qilái. Gèng duo de shíhòu, women zài 1702 Glenwood Avenue de lóu shàng danwèi zài chuángshàng zuò'ài. Xila youshí zài nàli guòyè. Women keyi zài wanshàng háishì qingchén zuò qìyue.
Wo de l?shi, ránhòu zàicì xiàng gaodeng fayuàn qisù. Zhè yicì, duìfang rènzhen duìdài shìqíng. Zài wo tóngyì qiaole yiqian meiyuán hòu, wo gei qizi hé wo de cáichan fenpèi shénme, ta tóngyì zhèxie tiáojiàn. Ta baoliúle báixióng hú de cáichan, ér zài 1991 nián zhiqián, wo geile wo $ 32,750 - $ 9,000, yú'é cóng 1991 nián kaishi, yi bai fen zhi qi de lì shuài cóng 1991 nián kaishi. Shìshí shàng, ta ji nián hòu jiù tíngzhi zhifù zhè bi piào, érqie wo bùdé bù ba zhè bi cáichan de liúzhì quán liú gei dangshí dào qí de jin'é. Dang ta zài 2000 nián chushòu fángchan shí, jíshi wo hái qiànle qián, suoyouquán de zhuanràng yeshì rúci.
Xila hé wo shì jiandìng de míngzì “pèi sen”. Women juédìng, rúguo shì nánhái, zhège míngzì jiùshì “yuehàn·pèi sen·mài gao gé” rúguo yigè nuhái “Jean Payson McGaughey”. Chaosheng biaomíng women de baobao shì nuxìng. Yinci,Sheila zài huáiyùn qíjian ganjué dào ta dùzi li de yùndòng, yizhí tí dào “xiao pàisheng”, you shíhòu hái huì tí dào “zhenni”. Dì èr gè míngzì ràng wo xiangqile wo zuì xihuan de Elton John gequ zhi yi.

However, the official belief of ‘sole responsibility for one’s own profits or losses’ that has been established since the marketisation of Chinese media industries made it impossible for the Fujian press industry to gain sustainable financial investments from the state. Hence, the present objective of media convergence has been to explore new pathways towards profitability. To date, only a few new media outlets in the Fujian press industry have garnered gains. This ‘adventitious feebleness’ in profitability is deemed by local journalists as a consequence of the ‘congenital deficiency’ in content as exacerbated by the censorship system.

Xi lì yà zài shèng ào lafu xiangshòuguò ta de suìyuè. Jian ér yán zhi, ta you yigè zhongguó nán péngyou gei ta yi zhi húlí gou de xiao gou zuòwéi shengrì liwù. Wèile wo de kuàilè, gou zài wo de guanxin zhong jiéshùle. Liánméng bù zànchéng nán péngyou, yinwèi ta ye duì qíta nurén shuo. Ta hé xi lì yà hen kuài jiù fenshoule. Jù wo suo zhi, ta hái méiyou rènzhen duìdài rènhé rén.
Wo zài 5 yuè 11 rì huí meiguó. Zài dì èr tian, wo da diànhuà gei xila wèn wo shìfou keyi duìxiàn ta 834 meiyuán. Ta shuo ta youshì yào gàosù wo. Shouxian, ta zài fùguó yínháng shiqùle gongzuò. Ta bèi gùyòngle liánbang jìhuà, ránhòu fàngqì, ta rènwéi, zài yínháng shou dào xiangguan de shuìshou youhuì hòu. Dì èr gè gèng zhòngyào de shì, ta xiànzài huáiyùnle. Ta qù kàn yisheng bú shufú, bìngqie liaojie dào ta de bùshì de yuányin. Xila yòng huáiyùn bao lái dào wojia. Ta zài yigè píngzi li jìnshí, wo ba huàxué tú céng de gùnzi fàng rù liúti zhong. Shì de, cèshì xianshì ta huáiyùnle.
Wang Xizhi is particularly remembered for one of his hobbies, that of rearing geese. Legend has it that he learned that the key to how to turn his wrist whilst writing was to observe how geese moved their necks. There is a small porcelain cup depicting Wang Xizhi "walking geese" in the China Gallery of the Asian Civilisations Museum in Singapore. The other side of the cup depicts a scholar "taking a zither to a friend".
Zhè wèi nushì, céng rèn jiudiàn zong jingli, xiànrèn zhongguó dì yi jia guangdà gongsi jiudiàn yèwù bùmén. Ta 43 suì. Ta de shí ji suì de nu'ér zài yingguó xuéxíle yi nián. Suirán liánhé pìnqingle kouyì yuán, dàn women nénggòu zài yingyu huìhuà zhong xianghù lijie. Lìrú, ta gàosù wo zhongguó nurén hen róngyì shìyìng zhàngfu de qíngkuàng. “Rúguo jià gei yi zhi ji,(qizi) shì yi zhi ji. Rúguo jià gei yi zhi gou,(ta) jiùshì yi zhi gou,“ta shì zenme shuo de. Zhè ye gei wo liú xiàle shenkè de yìnxiàng, lián nénggòu cóng méiyou ruanmù luósi de pútáojiu píng zhong quchu ruanmù sai - zhè shì jiudiàn guanli shìyè zhongxué dào de yigè qiàomén. Ta sìhu shì yigè màoxian de, you cáihuá de nurén.
Liù nián qián, wo faxiàn zìji chuyú jiéhun zhuàngtài, dàn xianrán wúfa yu wo xiànzài de qizi yiqi sheng háizi. Wo kaishi rènshì dào, zài wo de qíngkuàng xià, hunyin shì yigè zuzhi wo chéngwéi fùqin de jigòu, ér bùshì yigè cùjìn fùqin shenghuó de jigòu. Zhè bùshì duì hunyin yinggai shì shénme de waili? Wo yiwéi shì zhèyàng, suoyi wo ba shìqíng jiao geile wo zìji de shou. Wo kaishi xúnzhao jihuì chéngwéi hun wài de fùqin. Jianchí shèhuì gongyue duì wo lái shuo yìyì bù dà. Wo shengmìng zhòng shèng xià de shíjian youxiàn. Wo bùdé bù caiqu xíngdòng.
Liánxù 10 yuè 18 rì xingqí yi, wo hé wo du fei huíle míng ní abo lì si. Women cóng tongcháng dì lùndian kaishi. Hòulái, dang wo hé wò'erma yiqi qù gòuwù, ránhòu zài chaojí dà zìzhùcan zhong chi wufàn shí, women de guanxì dédào gaishàn. Liánxiang yào wo gèng duo de guanzhù ta. Yiqi qí che shì jijí de jinglì. Ta yú 11 yuè 2 rì xingqí yi huí dào zhongguó, zhè shì dàxuan dàngtian.
Zài 1973 nián wo jiéhun ka luó er zhiqián, wo yu yigè míng jiào zhu dí de niánqing nuzi youzhe jiqíng de guanxì, chíxùle haoji gè yuè. Ji nián hòu, wo zàicì tongguò xiànzài zhù zài jialìfúníya zhou de fùmu yu ta liánxì. Zài 1987 nián de dongtian, zhu dí da diànhuà shuo ta xiang bàifang wo. Ta bàoyuàn ta xiànzài de nán péngyou dài fu, yigè zài xingqiú dàzhàn xiàngmù shàng gongzuò de shùxué jia. Zhè dangrán shì duì wo yu an dì guanxì zàochéngle yalì. Bùguò, wo huanyíng zhu dí de fangwèn, bìng zhunbèi ràng ta zài bèiyòng fángjian dòuliú ji tian. Zhu dí dàodá jichang yigè dà shùgàn. Xianrán zhè cì fangwèn jiang chíxù yiduàn shíjian.
Specifically, information released in new media outlets emphasise timeliness. The irreconcilable conflict between such requirement and the principle of accuracy in news reportage concerns many journalists. In China, the common rule of thumb in terms of news production is that ‘the writer is responsible for the consequences of this article’. Journalists are obliged to take full responsibility for all issues engendered by the news under their name. The risk of ‘more mistakes with more releases’ is a shadow that follows news production as a by-product of the censorship system. Thus, the journalists in Fujian’s press groups disregard the new system of censorship as a ‘buffer’ that could protect them from various risks, particularly political ones. When journalists believe that the risk they may undertake outweighs the financial return they will attain, they choose not to cooperate.
C9-ALS iMNs were cultured with 10 nM inactive 3K3A-APC or 3K3A-APC for 3 days and then Hb9::RFP+ iMNs were flow purified. 3′-Digital gene expression RNA-Seq of all samples was performed by Amaryllis Nucleics. Briefly, mRNA was extracted using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer’s instructions. 3′ RNA-Seq libraries were generated using the 3′-Digital Gene Expression RNAseq Library Kit (Amaryllis Nucleics). Libraries were sequenced on an Illumina NextSeq 500 sequencer. A total of 10 million to 25 million 80-bp, single-end reads were obtained for each sample. Reads were aligned to the Hg38 transcriptome using HISAT2 (41). A count table was obtained using FeatureCounts (42) with strand specificity enabled. Differential expression analysis was performed using DESeq2 (43). The DESeq2 sample description table used, count table, and DESeq2 results can be found in the supplemental data. The sequencing data have been deposited in NCBI’s Gene Expression Omnibus (GEO) and can be accessed using GEO Series accession number GSE133162.
Before and after 2006, Chinese scholars began exploring the potential effects of ‘media convergence’ on news production in the context of digitisation being launched pervasively among press groups. A few of these scholars believe that convergence journalism, as an outgrowth of media convergence, provide gradual changes to the actors of news production, news source, communication medium, content, form and workflow (Cai, 2006).
Jinguan huáiyùn, xila hái you qíta xìngqù. Ta kaile yijia míng jiào Hopeffin de fei yínglì jigòu, wèi kùnrao de qingshàonián tígong zhùfáng. Lìngwài yijia chuàngyè gongsi shì Writersandphotographers,Inc., Yijia jiyú wangluò de yèwù, keyi ràng zhengzhá de zuò jia hé shèying shi zàixiàn shàng zhanshì zìji de sùcái. Wo xiang zhidào zhèyàng de qiyè rúhé zhuànqián. Xila xiàng wo baozhèng, ta keyi dédào buzhù jin. Ta hé ta de nu'ér lái nà zài míng ní abo lì si beibù kaile yijia xiaoxíng fúzhuang diàn, ránhòu zhèngzài názhe nápòlún shan dúshu. Wo de zhìxún shì fùmiàn yingxiang.
Quantification of the effect of TMX2 reduction by shRNA lentiviral transduction in two independent control iPSC lines, as performed in Fig. 7. Induced motor neurons were generated from iPSCs using a seven factor (7F) differentiation system. The survival of HB9-RFP+/shRNA-GFP+ iMNs was tracked by imaging after the addition of 10 µM glutamate to the cultures. All iMN survival experiments were analyzed by log-rank test, and statistical significance was calculated using the entire survival time course. *p < 0.05, ** p < 0.01 (survival assays were performed in triplicate with the indicated number of iMNs analyzed for each group, Supplementary Table 4).
Xila fei wang fóluólidá zhou jìn háng háng dòng. Women zhi néng tongguò diànzi yóujiàn jìnxíng goutong, ér bù shì diànhuà goutong. Yóuyú mou zhong yuányin, ta bùdé bù baoliú éwài de yitian. Wo zhèngzài shou dào miáoshù ta zài tíngche chang kàn dào de xiyì de diànzi yóujiàn. Zuìhòu,2007 nián 1 yuè 22 rì, wo shou dàole láizì xila de lìng yi feng diànzi yóujiàn, ta xuyào wo ba 2,320 meiyuán fàng rù zhipiào zhànghù, yibiàn éwài shouqu yiyuàn fèiyòng bìng fanhuí jiayuán. Bù, dang ta huílái shí, wo zài jichang yùjiàn bùliao ta.
The Chongzhen Emperor (Chinese: 崇禎; pinyin: Chóngzhēn; 6 February 1611 – 25 April 1644), personal name Zhu Youjian (Chinese: 朱由檢; pinyin: Zhū Yóujiǎn), was the 17th and last Emperor of the Ming dynasty as well as the last Han Chinese to reign as Emperor of China. He reigned from 1627 to 1644. "Chongzhen," the era name of his reign, means "honorable and auspicious".
*  “Zhutixing de lisanhua: Zhongguo xinyimin zuozhe zai Xinjiapo”  主体性的离散化:中国新移民作者在新加坡  (Diasporization of Subjectivity: New Chinese Migrant Writers in Singapore), presented at the Fifth Malaysia International Sinology Conference on “Sinology in the Globalization Era,” organized by the Faculty of Modern Languages and Communication, Universiti Putra Malaysia, Serdang, Malaysia, 12-13 September 2008.
“Zhuandong luopan: Chen Jiageng he Li Guangqian de huaren lisan jingyan” 转动罗盘:陈嘉庚和李光前的华人离散经验 (Turning The Compass: Diasporic Chinese Experience of Tan Kah Kee and Lee Kong Chian) in Leo Suryadinata (ed.), Tan Kah Kee and Lee Kong Chian in the Making of Modern Singapore and Malaysia.  Singapore: Chinese Heritage Centre: Tan Kah Kee Foundation, National Library Board, 2010, pp. 139-150.

Xi lì yà zài shèng ào lafu xiangshòuguò ta de suìyuè. Jian ér yán zhi, ta you yigè zhongguó nán péngyou gei ta yi zhi húlí gou de xiao gou zuòwéi shengrì liwù. Wèile wo de kuàilè, gou zài wo de guanxin zhong jiéshùle. Liánméng bù zànchéng nán péngyou, yinwèi ta ye duì qíta nurén shuo. Ta hé xi lì yà hen kuài jiù fenshoule. Jù wo suo zhi, ta hái méiyou rènzhen duìdài rènhé rén.

65 The coefficient of the interaction term was not statistically significant because of the small n of the treatment group. This indicates that we cannot be 95% certain that we would retrieve similar results over repeated samples. However, the dynamics are similar when comparing Beijingers' use of the internet and newspapers, thus further providing evidence that “new” media are more effective than “old” media in appeasing citizens. See Stockmann, “What kind of information does the public demand?”
Wúlùn shì zhongzú háishì qíta yuányin, wo de fùmu dou bù zànchéng wo yu xila de hunyin. Wo de muqin anpái wo de dìdì an dí hé 1960 nián bìyè de ai kè sai tè zhunbèi xuéxiào de yiqún xiàoyou yiqi qù zhongguó luxíng. ai kè sai tè bìyè sheng de luxíng tuán dangshí shì meiguó zhù huá dàshi de tian'anmén guangchang dà túsha. Wo bèi yaoqiú péibàn an dí, yi quèbao ta fúyòng ta di yàowù. Zhè cì luxíng yú 1996 nián 4 yuèdi kaishi, chíxùle san gè xingqí. Dang wo huí dào míng ní abo lì si shí, xila hé háizimen yiqi zoule, hái you dà píngmù diànshì hé qíta de cáiwù. Dang wo da diànhuà gei shànghai, méiyou rén huídá de shíhòu, wo you yigè zhèyàng de qíngkuàng.
Kenéng cóng luxíng zhong shòuyì de lìng yigè rén shì xila de cháng zi, tuoní, ta shì yigè bangpài chéngyuán. Yigè yuè hòu, tuoní bù xiaoxin zài míng ní abo lì si beibù de yitiáo xiao xiàng li qiangshale yi míng niánqing nuzi, dangshí ta cóng yi míng bèi duìshou bangpài chéngyuán zhànling de yi liàng qìche kai qiangdàn qilái. Tuoní de fal? biànhù wo ná chule yi wàn meiyuán. Nà yi nián,1995 nián, dangnián you zhème duo de móusha shìjiàn, women de chéngshì bèi cheng wèi “sharén zhe”. Tuoní yin èr dù móusha zuì bèi dìngzuì, bìng zài zhou jianyù bèi pàn shíwu nián túxíng. Ta shì míngnísudá zhou de dì yigè shàonián, yào chéngwéi yi míng chéngnián rén.
Lín dá duì zìji zài xuduo bùtóng qíngkuàng xià de pingé gan hen gan xìngqù. Ta kenéng shì yigè nu tóngzhì míni míngrén, yigè you bàofù de geshou, yigè heirén de gònghédang rén hé yigè qiángdà de nánrén de qíngfù. (Ta de zhuancháng zhèngzài péiyang yu NFL zhu jiàoliàn hé gao jíbié zhèngzhì jia de guanxì.) Ta zài bùshí 41 suì de shíhòu chéngwéi báigong shíxí sheng, dànshì dang ta tóusù zhongzú qíshìshí, ta bèi fàngkuanle zhège zhíwèi. Zài women de diànhuà zhong, ta shuo ta ganggang yù dàole kèla lún si·tuomasi. Tongguò ta, youxìng fangwènle yixie zhimíng rénshì dì gèrén shenghuó.
(A) Immunofluorescence images showing NR1+ punctae on neurites of iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 6 days. Scale bar: 2 μm. This experiment was repeated 3 times with similar results. (B and C) NR1+ punctae per unit area in control, C9-ALS (B), or sporadic ALS (C) iMNs. Each gray circle represents the number of NR1+ punctae per area unit on a single neurite (1 neurite quantified per iMN). n = 33 (controls and C9-ALS) or 13 (sporadic) iMNs quantified per line per condition from 2 biologically independent iMN conversions of 2 CTRL, 2 C9-ALS, or 6 sporadic ALS lines. Median ± interquartile range. Kruskal-Wallis testing. (D) Number of calcium transients per 30 seconds in control or C9-ALS iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC. n = 21 iMNs per line per condition from 3 biologically independent iMN conversions of 3 CTRL and 3 C9-ALS lines. For the C9-ALS plus 3K3A-APC condition, n = 19 iMNs per line. Median ± interquartile range. Kruskal-Wallis testing. (E) Number of calcium transients per 30 seconds in control or sporadic ALS iMNs treated with inactive 3K3A-APC or 3K3A-APC. n = 20 iMNs per line per condition from 3 biologically independent iMN conversions of 3 CTRL and 1 sporadic line. Median ± interquartile range. Kruskal-Wallis testing. (F) Immunoblotting of surface NR1 after surface protein biotinylation in C9-ALS iMNs generated with NGN2, ISL1, and LHX3 and treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 6 days. (G) Quantification of NR1 immunoblotting from F. n = 4 biologically independent iMN conversions. Each gray circle represents an individual sample. The ratio of surface to total transferrin receptor was used to normalize for the membrane protein extraction efficiency and TUJ1 was used to normalize for neuron number. (H) Immunoblotting of surface NR1 after surface protein biotinylation in sporadic ALS iMNs (1 patient) generated with NGN2, ISL1, and LHX3 and treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 6 days. The full blot for total TUJ1 is shown. (I) Quantification of NR1 immunoblotting from H. n = 4 biologically independent iMN conversions. Each gray circle represents an individual sample. The ratio of surface to total transferrin receptor was used to normalize for the membrane protein extraction efficiency and TUJ1 was used to normalize for neuron number. The day of differentiation stated on each panel indicates the day of differentiation on which the experimental treatment or time course was initiated. TF, transferrin.
Bùguò, huí dào meiguó, wo zài 2002 nián 9 yuè 8 rì shou dàole yi feng diànzi yóujiàn, nèiróng rúxià:“Wo hen yíhàn, zhidào ni de qizi shàng cì méiyou huáiyùn... Wo zài ci tígong yixie xìnxi kaol?. Shouxian, ni hé ni qizi de niánlíng dou bù xiao, huáiyùn de kenéng xìng huì hen di, dàyue shì 10%, ér ying'ér jixíng l? ye huì hen gao. Qícì, ni de jingzi de zhìliàng hé liúdòng xìng bùshì nàme hao, ni qizi de zigong nèi mó ye bùgòu hao... Dì san, ni qizi de jiànkang bù hao, you tángniàobìng hé gao xieya, huáiyùn huì èhuà zhèxie jíbìng. Shùnyì dì,IVF de fèiyòng chaoguò 20,000 yuán, chéngxù fùzá. Xiwàng yishàng xìnxi duì nín de juédìng shì youyòng de.“
Xila hé wo xiànzài you yigè xiao nánhái zhù zài women - dé'er huò “dài'er”, zhèngrú wo suo shuo de. Ta shì jianyù li de xila de érzi tuoní·senlín (Tony Foresta) de érzi. Tuoní yin móusha zuì bèi dìngzuì. Wo juédé ta shì wúgu de dài'er de muqin shì yi míng míng jiào sha yi dì meiguó bentu nurén. Ta zhù zài míng ní abo lì si nánbù de xiao dìqiú fùjìn. Dang xila quèdìng dài'er bèi hushì shí, ta jiben shàng bèi bangjià. Dài'er yizhí hé women yiqi shenghuóle dàyue liang nián. Women cóng sha yi shénme dou méi tingguò. Xila tánpàn zhèngshì caiyòng dài'er.
Oct. 2019: Trung Vu is awarded the Best Student Paper Award (second prize) at IEEE International Workshop on Machine Learning for Signal Processing, October 13-16, 2019 Pittsburgh, PA, USA! The award is given to a collaborative paper of Trung Vu, Raviv Raich and Xiao Fu titled ‘‘On Convergence of Projected Gradient Descent for Minimizing a Large-scale Quadratic over the Unit Sphere’’. Congratualations, Trung!
Consistent with our previous studies (4, 8), a 12-hour pulse treatment of 10 μM glutamate induced significantly faster degeneration of C9ORF72 patient iMNs compared with controls (Figure 1, B [individual lines shown] and C [iMNs from all control or patient lines combined into a single trace for clarity], and Supplemental Figure 1, J and K). Glutamate treatment was not strictly required to elicit this effect, as withdrawal of neurotrophic factor supplementation also selectively induced the rapid degeneration of C9ORF72 patient iMNs without glutamate treatment (Figure 1D and Supplemental Figure 1L). Thus, ALS-causing mutations can cause iMNs to degenerate significantly faster than those from controls.
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