Sep 2017: Try the Python implementation of our large-scale GCCA work ‘‘Efficient and Distributed Algorithms for Large-Scale Generalized Canonical Correlations Analysis’’ published at ICDM 2016. The implementation is by Adrian Benton at Johns Hopkins University, who has been doing interesting works in multiview analysis and natural language processing.
Because autophagy induction can enhance TDP-43 turnover in motor neurons (26), we wondered whether 3K3A-APC treatment could prevent the cytoplasmic accumulation of TDP-43 in ALS iMNs. 3K3A-APC treatment for 6 days significantly increased the nuclear-to-cytoplasmic ratio of TDP-43 in C9ORF72 and sporadic ALS iMNs to control levels (Figure 3, G–J; 2 controls, 2 C9ORF72 ALS, and 6 sporadic ALS patients). Thus, 3K3A-APC treatment can rescue autophagosome formation, lower DPR levels in C9ORF72 ALS iMNs and rescue DPR-mediated neurotoxicity, and reverse the cytosolic accumulation of TDP-43 in C9ORF72 and sporadic ALS patient iMNs.
(a) Sample distance matrix using hierarchical clustering with Euclidian distance metric of total transcriptome of each sample. (b) Principal component analysis of total transcriptome of each sample. (c) Clustered heat map showing normalized expression levels of top 40 genes sorted by adjusted p-value. All analyses were performed using DEseq2 in R (Supplementary Table 3).
Huíxiang qilái, wo hen yíhàn, wo méiyou gèng nulì de zhengjiù zhège háizi. anni de xiesidili - duì wo lái shuo keyi lijie - ràng wo yóuyù duì ta chéngnuò. Chusheng quexiàn de kenéng xìng ye feicháng guanxin wo. Wo de fùmu sìhu juédé duòtai shì zhèngquè de dàolù. Suoyi wo ganguò, jì bù gei an anwèi, ye bùyào xíngróng wo xiang yào zuò de qingxi huàmiàn. Zuìhòu, an dì zhèngyì xiongdì ba shìqíng jiao zài zìji shouzhong, wèi women zuò chule juédìng.
Ho Khai Leong 何启良 (ed.). Kuangzheng yu liubian: Malaixiya huaren lishi yu renwu zhengzhipian 匡正与流变：马来西亚华人历史与人物政治篇 (Malaysian Chinese History and Personalities: The Political Elites). Kuala Lumpur: Centre for Malaysian Chinese Studies, 2003; Hou Kok Chung 何国忠(ed.), Jicheng yu jueze: Malaixiya huaren lishi yu renwu wenhuapian 继承与抉择：马来西亚华人历史与人物文化篇 (Malaysian Chinese History and Personalities: The Intellectual Elites). Kuala Lumpur: Centre for Malaysian Chinese Studies, 2003; Lim Chooi Kwa 林水豪 (ed.), Chuangye yu hugen: Malaixiya huaren lishi yu renwu rushangpian 创业与护根：马来西亚华人历史与人物儒商篇 (Malaysian Chinese History and Personalities: The Entrepreneurial Elites). Kuala Lumpur: Centre for Malaysian Chinese Studies, 2003. In Xin Jiyuan Xueyuan xuebao 新纪元学院学报 (New Era College Academic Journal) Vol. 2 (April 2005): 169-171.
The cDNA for each iMN transcription factor or the mRFP-GFP-LC3 construct was subcloned into the pMXs retroviral expression vector (4). The Hb9::RFP lentiviral vector was previously purchased from Addgene (ID: 37081). Viruses were produced as follows. HEK 293T cells (ATCC, CRL-11268) were transfected at 80% to 90% confluence with viral vectors containing genes of interest and viral packaging plasmids (PIK-MLV-gp and pHDM for retrovirus, pPAX2 and VSVG for lentivirus) using polyethylenimine (Sigma-Aldrich). HEK 293T were tested for mycoplasma before, during, and after the study and were negative. Viruses were harvested at 48 hours and 72 hours after transfection. Viral supernatants were filtered with 0.45-μm filters, incubated with Lenti-X concentrator (Clontech) for 24 hours at 4°C, and centrifuged at 1500 g at 4°C for 45 minutes. Pellets were resuspended in 300 μL DMEM plus 10% FBS and stored at –80°C.
(a) Distribution of sequencing from 10 KCNK5 sgRNAs (blue) compared to distribution of sequencing counts of all negative control sgRNA (grey) from PR20 screen replicate 1 (this is an example of a protective genetic modifier). (b) Distribution of sequencing from 10 RHOA sgRNAs (red) compared to distribution of sequencing counts of all negative control sgRNA (grey) from PR20 screen replicate 1 (this is an example of a sensitizing genetic modifier). Using CasTLE these sgRNA count distributions were used to generate effect scores, confidence scores and p-values for each gene (see Supplementary Table 1). (c) Volcano plot of effect vs. confidence scores for all human genes in the PR20 screen. Colored in blue are all the genes conferring resistance to PR20 when knocked out (10%FDR) and colored in red are all the genes conferring sensitivity to PR20 when knocked out (10% FDR). (d) Correlation of casTLE scores for all genes in the library in our screen for modifiers of PR20 toxicity compared to an independent screen for modifiers of another toxic protein, ricin performed in near identical conditions (same lab, same cell line, same sgRNA library). There was zero (R2 = 0) overall correlation between the hits for each screen suggesting a high degree of specificity in the modifiers identified in each screen. Note that TMX2 was only identified as a hit in the PR screen and not the ricin screen.
Lā Qiáolā (La Jolla), Jiālìfúníyàzhōu -- Wénlín Yánjiūsuǒ Shèhuì Mùdì Gōngsī (SPC), Wénlín Hànyǔ Xuéxí Ruǎnjiàn 4.2 bǎn fāxíng gōngsī, xuānbù chénglì dì-yī rèn dǒngshìhuì. Dǒngshìhuì chéngyuán bāokuò: Xiàwēiyí Dàxué Mǎnuò'ā Xiàoqū Zhōngguó Yánjiū Zhōngxīn fùzhǔrèn, Kǒngzǐ Yánjiūsuǒ zhǔrèn Rèn Yǒuméi (Cynthia Ning) bóshì; Shèngdìyàgē Zhōulì Dàxué Zhōngwén Xiàngmù gùwèn, “Měiguó Zhōngwén Jiàoshī Xuéhuì Zázhì” zhǔbiān Zhāng Zhèngshēng (Zheng-sheng Zhang); Richard Cook bóshì (yánjiū yǔ kāifā fùzǒngcái); Elisabeth Nuboer-Ranjhan (zhànlüè guānxì fùzǒngcái); Mark Roblee（yíngxiāo fùzǒngcái) hé Tom Bishop (zǒngcái).
“Chongzu quanli yu huangui xingshi: Nanda 1964 zhi 1968 nian de gaizhi yu gaige” 重组权力与换轨行驶：南大1964至1964年的改制与改革 (Reconfiguring Power and Changing Track: The Changes and Transformation of Nanyang University, 1964-1968), presented at Nantah History Workship on “Zuqun, jiaoyu, guojia: 1953 zhi 1980 nian de Nanyang Daxue” 族群、教育、国家：1953至1980年的南洋大学 (Ethnicity, Education, and Country: Nanyang University, 1953-1980), in Singapore organized by the Centre for Chinese Language & Culture, Nanyang Technological University, 5 August 2006.
Zài qízhong yigè changhé, wo gàosù xila, wo shì tú zhaodào yigè méiyou jiéhun de nurén, yào hé wo yiqi shenghuó. Ta de guanzi bèi shùfù hòu, ta wúlì. Rán'ér, dang women jiéhun de shíhòu, women yijing jiandan dì wéi ta zuòle yigè shoushù lái niuzhuan shuluanguan jiéza. Zì nà yilái, zhè xiàng jìshù jìnbùle. Xila zài hùliánwang shàng jìnxíng sousuo, zài fóluólidá zhou de kèlì er wò tè zhaodàole yigè dìfang, jiang yi 10,000 meiyuán jìnxíng nìzhuan chéngxù. Ta rènwéi, boshìdùn kexué gongsi de jiànkang baoxian kenéng huì zhifù bùfèn chéngben. Rúguo you xìngqù, wo jiang bùdé bù zhifù qíyú fèiyòng.