Huísù zhìshao wuqiannián, dang wénmíng shèhuì shoucì chuxiàn shí, yuánlái de wénhuà zhuanbiàn fashengle. Zhè zhong qíngkuàng shouxian fasheng zài sànbù de chéngshì - su mei er de zhongxin dìdài, ye cheng wèi mei suo bù dá mi yà, zhihòu zài aijí. Zhongguó, yìndù hé maya rén zài yihòu de shíjian jinglìle xiangtóng de guòchéng. Xiezuò de fa míng bànsuízhe chéngshì shèhuì de xingqi. Zài ci zhiqián, rénlèi wénhuà shì jiyú koutóu dehuà. Zhishì bìxu bèi jì zhù, gèrén hé jíti, chéngwéi wénhuà de yibùfèn. Bùluò zhanglaomen jiang koutóu mínjian chuánshuo chuán geile tamen cóng zìji de zuxian shenshang xué dào de xin yidài. Yu xiezuò lái yi zhong dúlì de fangfa jì zhù zhèyàng de shìqíng. Kao niántu huò shítou zhong de biaojì xìngcún yú kè you tamen de rén. Zhiyào yourén zhidào rúhé jieshì shumiàn fúhào, tamen de zhishì jiù huì jìxù cúnzài.
“Mahua wenxue de zuqunxing: yanjiu lingyu de jiangou yu wuqu” 马华 文学的族群性:研究领域的建构与误区 (Ethnicity of Mahua Literature: Construction and Pitfalls of A Research Field), presented at the Third Asia Forum, Historical and Cultural Studies on “Ethnic Relations and Regional Comparison of the Chinese Communities,” in Singapore, organized by the Department of Chinese Studies, National University of Singapore, 13-15 November, 2009.
(a) TMX2 protein reduction measured by immunoblot in K562 cells expressing Cas9 and sgRNAs targeting TMX2 or control sgRNAs (GAPDH levels were measured as loading control). (b) Enrichment of Tmx2 KO cells compared to WT in a K562 competitive growth assay after synthetic PR20 treatment. Cells measured 48 h after 10 µM PR20 treatment by flow cytometry. (c) Sample distance matrix using hierarchical clustering with Euclidian distance metric of total transcriptome of each sample. (d) Principal component analysis of total transcriptome of each sample. (e) Differential gene expression was determined using DEseq2, and the top differentially expressed genes as ranked by adjusted p-value were clustered using the R package pheatmap. Color indicates relative log2 fold change in normalized read counts from DEseq2. MA plot showing abundance of differentially expressed genes in red (adjusted p-value < 0.05) for pairwise comparisons of (f) control sgRNA neurons expressing PR50 vs GFP, (g) Tmx2 KO neurons expressing PR50 vs GFP, and (h) Tmx2 KO neurons expressing PR50 vs control sgRNA neurons expressing PR50 (Supplementary Table 3).
The recruitment of interviewees was conducted for two rounds. Given the demonstrative effect of the media convergence of the Xiamen Daily Group within the province and even the entire industry, the authors contacted seven media practitioners from this organisation to undergo the first round of interview. Consequently, one director, one editor and five newspaper journalists were involved as interviewees in this round. After developing a preliminary knowledge of the opinions of the Xiamen Daily Group journalists towards media convergence, the authors conducted a second round of interview. Three directors, three editors and seven journalists were recruited as interviewees from several other press groups, including Fujian Daily Group, Fuzhou Daily News Press and Quanzhou Evening News Press, thereby promoting the diversity of the interviewees in the aspect of age, years at work and newspaper type. Except for one journalist from the Fujian Daily Group who was interviewed via telephone, all the 19 others underwent face-to-face interviews (Table 1).
In light of the advanced international conception for e-learning translation instruction and the development of professional translation education in China, it is proposed that an e-learning platform conceived with knowledge systems be constructed. The new platform aims at utilizing advantages and characteristics of the online instruction, and at the same time categorizing instructive resources leading to knowledge service systems. This advantageous e-learning environment is featured with a collection of electronic resources of translation technology, instructive resources and interactive communication forums.
Dang xi lì yà jìxù jieshòu jiàoyù de shíhòu, lián sìhu bijiào... Ta céngjing zài zhongguó gao shuipíng yanchu, dàn yóuyú yingyu shuipíng bùzú, duì meiguó wénhuà de wúzhi, ta gandào hen gangà. Wo méiyou àn ta fù qián gongzuò. Yinci, ta jingcháng zuò zài diànnao qián wán yóuxì. Wo tóngshí zhuiqiú yi zhèngzhì, xiezuò, fángdong zuzhi hé guanli zulìn wùyè wéi zhongxin de zìji de lìyì. Women bèi kùn zài women fenkai de shenghuó zhong.
Dànshì sixiang you yixie jìqiao lái jiaqiáng hé baoliú jì zhù de zhishì. Shouxian, yijing chóngfùle ji cì de koutóu biaodá qingxiàng yú bi zhi shuo yicì de dongxi jì zhù gèng duo. Yinci, bùluò zhang zhe tongguò chóngfù gùshì qiánghuà tamen de koutóu wénhuà. Dì èr, kouyu jiaoliú kenéng bàn you túpiàn, míngkè de fúhào, yíshì wudao, huò qíta wùti de wùli wàiguan chùfa jìyì. Dì san, koutóuyu keyi yi guizé móshì anpái, zài qízhong gòujiàn jìyì. Zài shige zhong, shiyòng jì fèi qì, yayùn hé gupiào duanyu bangzhù qí jìyì zhe jì zhù suo shuo huàyu de tèdìng duàn.
3K3A-APC potently rescued autophagosome formation in C9ORF72 ALS iMNs, as determined by its ability to increase the number of GFP+mRFP+ vesicles in bafilomycin-treated iMNs (Figure 2, A and B [C9-ALS, inactive 3K3A-APC, +bafilomycin vs. C9-ALS, 3K3A-APC, +bafilomycin]; 3 controls and 3 C9ORF72 ALS patients). Interestingly, 3K3A-APC also increased autophagosome formation in sporadic ALS iMNs (Figure 2, A and C [sporadic ALS, inactive 3K3A-APC, +bafilomycin vs. sporadic ALS, 3K3A-APC, +bafilomycin]; 3 controls and 5 sporadic ALS patients, and Supplemental Figure 2B), indicating that is also capable of rescuing C9ORF72-independent autophagy impairments. Western blot analysis of C9ORF72 ALS motor neurons showed that 3K3A-APC increased the ratio of LC3-II to LC3-I in the presence of bafilomycin, verifying the results of the mRFP-GFP-LC3 assay (Supplemental Figure 2, C and D). Therefore, 3K3A-APC treatment rescues autophagosome formation in C9ORF72 and sporadic ALS iMNs.
La Jolla, Jiāzhōu — Wénlín Yánjiūsuǒ yǒuxiàn gōngsī (SPC) zhèngshì fābù Wénlín Hànyǔ Xuéxí Ruǎnjiàn 4.3 háohuá bǎn (Wénlín Wánzhěngbǎn). Xīn fābù de 4.3 bạ̌nběn wánquán zhīchí wánzhěngbǎn Hànyǔ Dà Cídiǎn suọ̌yǐn, suọ̌yǐn jīngguò gēngzhèng, kuòchōng bìng àn zìmǔ shùnxù páiliè, zuòwéi fùjiā chéngxù kě yǔ Wénlín ABC cídiǎn wánměi rónghé, jǐn shòu 49 Měiyuán. Wénlín ABC HDC suọ̌yǐn ruǎnjiàn bǎn kě ràng yònghù qīngsōng chákàn HDC ruǎnjiàn zhōng dàiyǒu de 350, 000 ge duōyīnjié tiáomù. Wénlín ABC HDC kuòzhǎn chéngxù shì yī zhǒng xiānjìn de yánjiū gōngjù, gòng shōunàle chāoguò 568, 000 ge tiáomù. Tōngguò cānkǎo HDC tiáomù, nǐ kẹ̌yǐ gènghǎo de lị̌jiě hěn duō ABC dāncí (bụ̀lùn shì-fǒu chūxiàn zài HDC). Tóngyàng de, HDC hángàile xǔduō wèi shōulù zài ABC cídiǎn de tiáomù, dàn yǔ ABC cíkù jiéhé qǐlai huì gènghǎo lị̌jiě. ABC+HDC de cházhǎo gōngnéng jīngcháng huì dǎoxiàng xiāngguān xìnxī, wúlùn shì Wénlín zhíjiē lièchū de liànjiē huòshì tōngguò Wénlín xīn tuīchū de wǎngluò sōusuǒ gōngnéng. HDC suọ̌yǐn yǔ ABC Hànyǔ cídiǎn xìliè zhǔbiān Méi Wéihéng (Victor H. Mair) jiàoshòu, (láizì Bīnxīfǎníyà dàxué), xiě dào︰ "Kàndào Wénlín fābùle yǐ dānxiàng páixù zìmǔ suọ̌yǐn wánzhěngbǎn «Hànyǔ Dà Cídiǎn» de diànzǐ bǎn, wǒ fēicháng gāoxìng. Zhè duì chīmí Hànyǔ yánjiū de měi yī gèrén lái shuō quèshí shì yī ge fúyīn".
“Locating Chinese New Migrants in National and Ethnic Contexts: The Singapore Case,” presented at the book launch of Lee Guan Kin (ed.), Demarcating Ethnicity in New Nations: Cases of the Chinese in Singapore, Malaysia, and Indonesia 新马印华人:族群关系与国家建构(Singapore: Singapore Society of Asian Studies, Konrad-Adenauer-Stiftung, 2006)and public talk on “Culture and Nation: Ethnic Chinese in Singapore, Malaysia, and Indonesia” 新马印华人:文化与国家  at the National Library, Singapore, organized by Singapore Society of Asian Studies, Kondrad-Adenauer-Stiftung, and Singapore’s National Library Board, 17 December 2006.
To confirm our previous findings showing that iMNs derived from patients harboring ALS-causing genetic mutations display rapid neurodegeneration in vitro (4, 5, 8), we used transcription factor–mediated conversion (4, 13) to generate iMNs from fibroblast-like cells derived from 3 control and 3 C9ORF72 ALS patient iPSC lines that we previously established and characterized (Figure 1A, Supplemental Table 1, and ref. 4). To track individual iMNs, we labeled them using an Hb9::RFP lentivirus that accurately labels HB9+ motor neurons derived in this manner (refs. 4, 5, 8, Figure 1A, and Supplemental Figure 1C) and used robotic microscopy and longitudinal tracking of iMNs to measure their survival (ref. 4 and Figure 1B). The density of Hb9::RFP+ iMNs corresponded to an approximately 10%–25% conversion rate and was similar across the different control and C9ORF72 ALS lines, and iMNs showed consistent survival across the range of iMN densities used in our experiments (Supplemental Figure 1, C–E). The vast majority of neurons in iMN cultures were motor neurons, with only rare tyrosine hydroxylase+ (dopaminergic neuron marker) or CTIP2+ (cortical neuron marker) neurons detected (Supplemental Figure 1, F–I). iMN cultures were supplemented with primary mouse mixed glia to facilitate iMN maturation.
Born in Linyi, Shandong, Wang spent most of his life in present-day Shaoxing, Zhejiang and Wenzhou. He learned the art of calligraphy from Lady Wei Shuo. He excelled in every script but particularly in semi-cursive script. His representative works include, in chronological order, Narration on Yue Yi (樂毅論), The Yellow Court Classic (黃庭經), Commentaries on the Portrait of Dongfang Shuo (東方朔畫讚), Admonitions to the Emperor from the Imperial Mentor (太師箴), Preface to the Collection of Poems Composed at the Orchid Pavilion (蘭亭集序, also commonly known as Lanting Xu ), and The Statement of Pledge (告誓文).[2] Unfortunately, none of his original works remains today, and only models of them exist. Samples of Wang's handwriting can also be seen in classical Chinese calligraphy texts such as the Chunhua Imperial Archive of Calligraphy Exemplars (淳化閣帖).[3]
Despite the party-market corporatism, Lee et al. (2007) explained that significant multiplicity continues to exist in the developmental path of local media because of differences in power structure and market maturity. Guangzhou media represent the ‘market competition within party-state ideological limits’ pattern. For example, three press groups in Guangzhou compete fiercely with separate content strategies, while Guangzhou Daily and Yangchen Evening Daily cater primarily to daily life relevance and avoid ‘grand narratives’, Nanfang City Daily and Nanfang Weekend often expose official wrongdoings and advocate liberal ideas. Beijing media that are hierarchically structured in parallel to national, ministerial and municipal levels of administrative authorities compete both horizontally and vertically for power and market on behalf of their patrons and constituencies, thus represent the ‘managed diversity through a precarious of the emerging interest politics among counterbalancing power bases’ pattern. For example, as the authors put it, the supreme leader Mao had to turn to the Shanghai press to wage wars against the Beijing Daily on the eve of the period of the Cultural Revolution because his influence at that point could not fully reach the constituency of the Beijing Daily (Lee et al., 2007).

La Jolla, Jiāzhōu — Wénlín Yánjiūsuǒ yǒuxiàn gōngsī (SPC) zhèngshì fābù Wénlín Hànyǔ Xuéxí Ruǎnjiàn 4.3 háohuá bǎn (Wénlín Wánzhěngbǎn). Xīn fābù de 4.3 bạ̌nběn wánquán zhīchí wánzhěngbǎn Hànyǔ Dà Cídiǎn suọ̌yǐn, suọ̌yǐn jīngguò gēngzhèng, kuòchōng bìng àn zìmǔ shùnxù páiliè, zuòwéi fùjiā chéngxù kě yǔ Wénlín ABC cídiǎn wánměi rónghé, jǐn shòu 49 Měiyuán. Wénlín ABC HDC suọ̌yǐn ruǎnjiàn bǎn kě ràng yònghù qīngsōng chákàn HDC ruǎnjiàn zhōng dàiyǒu de 350, 000 ge duōyīnjié tiáomù. Wénlín ABC HDC kuòzhǎn chéngxù shì yī zhǒng xiānjìn de yánjiū gōngjù, gòng shōunàle chāoguò 568, 000 ge tiáomù. Tōngguò cānkǎo HDC tiáomù, nǐ kẹ̌yǐ gènghǎo de lị̌jiě hěn duō ABC dāncí (bụ̀lùn shì-fǒu chūxiàn zài HDC). Tóngyàng de, HDC hángàile xǔduō wèi shōulù zài ABC cídiǎn de tiáomù, dàn yǔ ABC cíkù jiéhé qǐlai huì gènghǎo lị̌jiě. ABC+HDC de cházhǎo gōngnéng jīngcháng huì dǎoxiàng xiāngguān xìnxī, wúlùn shì Wénlín zhíjiē lièchū de liànjiē huòshì tōngguò Wénlín xīn tuīchū de wǎngluò sōusuǒ gōngnéng. HDC suọ̌yǐn yǔ ABC Hànyǔ cídiǎn xìliè zhǔbiān Méi Wéihéng (Victor H. Mair) jiàoshòu, (láizì Bīnxīfǎníyà dàxué), xiě dào︰ "Kàndào Wénlín fābùle yǐ dānxiàng páixù zìmǔ suọ̌yǐn wánzhěngbǎn «Hànyǔ Dà Cídiǎn» de diànzǐ bǎn, wǒ fēicháng gāoxìng. Zhè duì chīmí Hànyǔ yánjiū de měi yī gèrén lái shuō quèshí shì yī ge fúyīn".
Mar. 2020, Undergraduate Research Assistantship Available: I am looking undergraduate research assistants in EECS at Oregon State University who are interested in statistical machine learning. Please send me your C.V. and transcripts if you are interested in working with me starting summer or Fall 2020 (or Winter 2021). The research experience program will typically be 10 weeks (one term).
Dàgài zài zhège shíhòu, xila hé wo you wú baohù dì xìng xíngwéi, dàgài zài 3 yuè zhongxún huòzhe wanqí. Women dangshí dou méiyou tài duo xiangfa. Wo bù rènwéi xila huì huáiyùn, yinwèi wo bù xiangxìn ta you shoushù lái niuzhuan shuluanguan jiéza. Xila shuo ta ye rènwéi, yinwèi ta de niánlíng, ta bù huì huáiyùn. Xìng shì women zuì hao de yibùfèn, dànshì, women rènwéi zhè shì quánbù.

Although the competitiveness of commercial new media are discerned, the interviewed journalists remain inclined to regard the contents of new media as ‘superficial’, ‘fast food-like’ and ‘targeting lower-level readers’. Accordingly, the journalists believe that the poor quality of the content provided by the nonprofessional new media outlets further highlights the importance of the journalists’ professional expertise of separating falsity from truth.
Lā Qiáolā (La Jolla), Jiālìfúníyàzhōu -- Wénlín Yánjiūsuǒ Shèhuì Mùdì Gōngsī (SPC), Wénlín Hànyǔ Xuéxí Ruǎnjiàn 4.2 bǎn fāxíng gōngsī, xuānbù chénglì dì-yī rèn dǒngshìhuì. Dǒngshìhuì chéngyuán bāokuò: Xiàwēiyí Dàxué Mǎnuò'ā Xiàoqū Zhōngguó Yánjiū Zhōngxīn fùzhǔrèn, Kǒngzǐ Yánjiūsuǒ zhǔrèn Rèn Yǒuméi (Cynthia Ning) bóshì; Shèngdìyàgē Zhōulì Dàxué Zhōngwén Xiàngmù gùwèn, “Měiguó Zhōngwén Jiàoshī Xuéhuì Zázhì” zhǔbiān Zhāng Zhèngshēng (Zheng-sheng Zhang); Richard Cook bóshì (yánjiū yǔ kāifā fùzǒngcái); Elisabeth Nuboer-Ranjhan (zhànlüè guānxì fùzǒngcái); Mark Roblee(yíngxiāo fùzǒngcái) hé Tom Bishop (zǒngcái).
All animal use and care were in accordance with local institution guidelines of the University of Southern California and the IACUC board of the University of Southern California with the protocol numbers 20546 and 11938. Wild-type C57BL/6J (strain: 000664), and C9-BAC (C57BL/6J-Tg(C9orf72_i3)112Lutzy/J, strain: 023099) were purchased from Jackson Laboratories. Mice were housed in standard conditions with food and water ad libitum in the conventional vivarium at the University of Southern California.
Dang wo huí dào yigè kong fáng zi shí, wo hen zhènjing. Xila hé háizimen zhèngzài bùlukè lín gongyuán zu yi jiàn gongyù. Jíshi xila hé wo jìxù you guanxì, women juédìng tíchu líhun. Women dou méiyou duì lìng yifang tíchu yaoqiú. Women anpáile yigè l?shi zhùli, qicaoliao dàyue 100 meiyuán de wénjiàn. Xila hòulái shuo, zài qianshu líhun falìng zhiqián, faguan wèn ta shìfou quèdìng ta bùxiang yào wo de cáichan. Xila méiyou, women zài 1996 nián 11 yuè líhunle. an dí jìxù zhù zài wo jiali. 1998 Nián qíngrén jié, ta zìji jiéhunle. Wo zài zhè duàn shíjian nèi shì danshen de.

Suoyi zhulì'an yizhí shì hui guniáng gùshì de nu zhujiao. Xiangxiàng yixià, wú jia ke gui, méiyou da lánqiú liang nián, ránhòu túrán jian, ta zài gaozhong bìyè, túpòle yóuxì de zhuzai. KARE-TV ye duì ta zuòle yigè gùshì. Yahu dá'àn wèn:“Shéi shì míngnísudá zhou zuìgao fen de gaozhong lánqiú yùndòngyuán?” Duìyú nuxìng qiúyuánlái shuo, zhè shì àidísheng gaozhong de zhulì'an. Dàn nà shì liang nián qián. Zhè tiáo lù yijing biàn lengle. 2008 Nián 3 yuè, zài bei dáketa zhou de yi suo dàxué, tiyù wangzhàn shàng youyi pian tí wèi “zhulì'an - zài VCSU wán de jixiàng” de gùshì. Méiyou jìnyibù de fa xiàn.
Wo zài yù zhong èrshísì xiaoshí, ránhòu huòzhun baoshì. Lín dá cóng jiali zoule. Dang ta yitianhòu hé ta liánxì, ta shuo ta xiang huí dào shènglùyìsi. Wo de fángjian, zàixiàmiàn dì dìban shàng de rán mù lúzi jiarè, tài lengle, bùnéng zài nàliguò dongtian. Lín dá huí dàole yigè qián nán péngyou. Si hòu, ta ban dàole danfú, zuìhòu jiéhunle yigè wéi dàxíng zuzhi zuò diànnao gongzuò de báirén. Tamen xiànzài zhù zài la si wéi jia si, lín dá zài zhoulì dàxué danrèn jiàoxué zhíwù.

As an alternative to the 7F iMN differentiation procedure used in Fig. 7 and S8, iMNs were differentiated from C9-ALS and control iPSC lines using a Dox-NIL system. (a, b) Survival of Dox-NIL iMNs with or without TMX2 reduction by shRNA transduction. Results from two control (a) or two C9-ALS (b) lines were averaged to create the survival curves shown. (c, d) The same iMN data depicted in (b) but separated by individual C9-ALS cell line to show the variability in responses. (e) Representative images of GFP+ (shRNA expressing) C9-ALS iMNs taken during the survival experiments. (f) RNA was harvested from iMN survival experiments at the endpoint and TMX2 mRNA levels were measured by qRT-PCR (normalized to GAPDH levels). For information on the patient lines used and numbers of iMNs analyzed for survival analysis, see Supplementary Table 4.

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