3K3A-APC also rescued the survival of iMNs from all 6 sporadic ALS lines (Figure 5H and Supplemental Figure 6, A–F; 6 sporadic ALS patients). When we tested iMNs from separate iPSC clones of the sporadic patient lines, 3K3A-APC also significantly improved their survival, indicating that the rescue effects were not specific to iMNs derived from certain iPSC clones (Supplemental Figure 6, G–J; 3 sporadic ALS patients). Reinforcing the notion that activating autophagosome formation in ALS iMNs could increase survival, rapamycin treatment significantly improved the survival of iMNs from 4 of 5 sporadic ALS lines tested (Supplemental Figure 7, A–J; 6 sporadic ALS patients). In contrast, rapamycin slightly decreased the survival of control iMNs (Supplemental Figure 7, K and L). These results suggest that rescuing autophagosome formation in sporadic ALS iMNs can increase their survival.
Yi wèi huífù de nushì zài yuehuì wangzhàn shàng tí dào wo de zhàopiàn. Wo bùnéng tongguò zhifù mei yuè huìyuán fèi jiarù wangzhàn lái chákàn túpiàn. Zhè shì wo duì hùdòng xìng wangzhàn de jièshào. Zuìhòu, wo jiarùle yigè míng wèi SexSearch.Com de wangzhàn, ránhòu jiarùle HornyMatches.Com. Wo xuanle yigè dàima míngcheng, jianyào jièshàole zìji, ránhòu kaishi huíying nàxie luoti túpiàn sìhu xiyin rén de nuxìng de guanggào.

Xila dangrán shì fènkai de. Hòulái ta gàosù wo, chao yinbo méiyou zài Cottage Grove zhensuo bèi caiqu, dàn zài míng ní abo lì si shì zhongxin de yiliáo jigòu. Ta huì anpái wo gen jianchá ta de yisheng shuohuà. Women shouxian xuyào tiánxie yu Allina de biaogé, ta jiang tóngyì pilù. Women zuòle liang cì. Shouxian, zhè xianrán bùshì zhèngquè de xíngshì. Ránhòu, yiyuàn jùjué tongguò diànhuà gàosù wo rènhé dongxi, yinwèi bìngrén de yinsi guidìng.


To encourage journalist participation, two newspaper offices in Fujian added ‘volume of news feeding to the centre’ in the their evaluation criteria for journalists’ work performance, and many other newspaper offices of the Fujian press industry stimulated the initiatives of journalists for collaboration with ‘remuneration’ (Gaofei) or ‘points’ (Gaofen). Motivated by these measures, a few journalists, particularly the young and junior ones, began to adapt to the working tempo of rapid publication and multiple ‘versions’ of a single story. Others, particularly old and senior journalists, had matter-of-fact reactions to such measures. From the perspective of the new media centre director and editors, the material rewards that failed to meet the expectations of journalists constitute the primary reason for the latter’s reluctance to participate. However, interviews with journalists revealed that their willingness to contribute news reports depends on the anticipated gains and the price that they may have to pay for such participation.
Zhang, ZA, Wu, T (2014) The dual declinings of propagandist and watchdog roles: Chinese Journalists’ media role perception, the changes and antecedents (‘Xuanchuanzhe’ yu ‘jianduzhe’ de shaungchong shiwei: Zhongguo xinwen congyezhe meijie juese renzhi, bianqian ji yingxiang yinsu). Journal of International Communication (Guoji xinwenjie) 6: 61–75.
Wo yiwéi jiéhun jiudiàn jingli de yìsi shì shuo, rúguo women bù tóngyì, wo de qizi huì shì wo keyi tuifan de rén. Shìshí zhèngmíng bùshì zhen de. Xiàng wo de dì yi rèn qizi, lián jingcháng ting qilái xiàng yigè pò jìlù de jìlù: Wo méiyou luxíng wo zhàngfu de zhízé. Wo cónglái méiyou gei ta “qizi de lìchang”. Wo shì zìsi de wo zhi xiang zìji qù xiangshòu. Dang wo duì wo de shengyin biaoxiàn chu rènhé fánnao shí, wo duì ta hen shengqì. Ta shengqìle you yicì, zài ting liánlián dehuà de shíhòu, wo juédìng bù qù zhenglùn, zhishì baochí chénmò. Wo shíwu fenzhong zuoyòu jiù gen wo shuole yijù huà. Wo gandào jingyà de shì, dang zhège sùsòng jiéshù de shíhòu, liánxì rén shuo zhè shì wo tingguò ta de ji cì zhi yi.
C9ORF72 ALS iMNs have fewer lysosomes than control iMNs (4), which could affect autophagic flux downstream of autophagosome formation. To determine if 3K3A-APC affects lysosome number in C9ORF72 ALS iMNs, we examined the number of LAMP2+ vesicles in iMNs (4). 3K3A-APC restored normal lysosome numbers in C9ORF72 ALS iMNs (Figure 2E; 2 controls and 2 C9ORF72 ALS patients, and Supplemental Figure 2, L and M). 3K3A-APC did not alter C9ORF72 protein (Supplemental Figure 2, N and O; 3 C9ORF72 ALS patients) or mRNA levels (Supplemental Figure 2P) in C9ORF72 ALS iMNs. Therefore, 3K3A-APC rescues lysosome numbers in C9ORF72 ALS iMNs without altering C9ORF72 levels.
Zhèxie wangzhàn de tao lún yuqì chúncuì shì ganxìng de. Xìng shì wèile chansheng shenti de lèqù. Zài mou xie qíngkuàng xià, kenéng huì daozhì ài yu hunyin. Rán'ér, yi shengzhí wèi mùdì dì xìngbié sìhu chaochule cangbái. Wo zuòwéi yigè xúnqiú lèqù de nánrén, ránhòu, rúguo shíjian sìhu zhèngquè, wo huì gaibiàn zhutí, jieshì wo zhenzhèng xiang yào de shì yigè ying'ér. Wo bù jìdé zhège cèlüè shì youxiào de. Yóuyú wo chéngnuò hé lián jiéhun, suoyi wo zhèngzài chuli yi zhi shou bang zài bèihòu. Zuìzhong, wo zhongzhile wo de huìyuán zigé. Wo huì jìxù shou dào lièbiao zhong de fùnu de diànzi yóujiàn, dànshì bùnéng zài bù gengxin huìyuán zigé de qíngkuàng xià huífù.

Shangjia kenéng yijing dì yi cì shiyòng ta. Tamen zài fangwèn mòsheng hé yáoyuan dì dìfang de jinglì cìjile yuyán, zongjiào, xísú hé sixiang zhi jian de bijiào. Zhège duan shouxie de jiaoben chuàngzàole yi zhong gèngjia jinglì chongpèi hé tànsuo de shèhuì lèixíng. Yixie xúnwèn zhe shì fazhan zìji de sixiang xìtong de zhéxué jia. Suízhe xiezuò zhishì guangfàn chuánbò, qí shìjué biaojì chéngwéi hàoqí xin de duìxiàng, yóuqí shì zài jùyou qiángliè koutóu chuántong de shèhuì. Zhè zhong shèhuì cúnzài yú 5 shìjìde xilà chéngbang. Hé ma shi, jì dé bù dú, shì xilà rén liánhé de wénhuà juzhèn. Rán'ér, xiàng Hesiod,Aeschylus hé Sophocles zhèyàng de zuòjia ye zài xiànchang.
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