Lā Qiáolā (La Jolla), Jiālìfúníyàzhōu -- Wénlín Yánjiūsuǒ Shèhuì Mùdì Gōngsī (SPC), Wénlín Hànyǔ Xuéxí Ruǎnjiàn 4.2 bǎn fāxíng gōngsī, xuānbù chénglì dì-yī rèn dǒngshìhuì. Dǒngshìhuì chéngyuán bāokuò: Xiàwēiyí Dàxué Mǎnuò'ā Xiàoqū Zhōngguó Yánjiū Zhōngxīn fùzhǔrèn, Kǒngzǐ Yánjiūsuǒ zhǔrèn Rèn Yǒuméi (Cynthia Ning) bóshì; Shèngdìyàgē Zhōulì Dàxué Zhōngwén Xiàngmù gùwèn, “Měiguó Zhōngwén Jiàoshī Xuéhuì Zázhì” zhǔbiān Zhāng Zhèngshēng (Zheng-sheng Zhang); Richard Cook bóshì (yánjiū yǔ kāifā fùzǒngcái); Elisabeth Nuboer-Ranjhan (zhànlüè guānxì fùzǒngcái); Mark Roblee(yíngxiāo fùzǒngcái) hé Tom Bishop (zǒngcái).

Fujian’s case indicates that new media outlets tend to be ‘domesticated’ in terms of converging news production. Firstly, a multilevel censorship system has been established, thereby ensuring that the new media content is under supervision. Secondly, new media outlets are subject to a bureaucratic style of management with low decision-making efficiency.
47 On 10 and 12 April, media briefings with Foreign Ministry spokesman Qin Gang were convened. See http://www.fmprc.gov.cn, accessed 31 May 2007. For reports see Beijing Youth Daily, 13 April 2008, Jinghua shibao, 13 April 2005, China Daily, 13 April 2005. Similar announcements by the spokesperson of the Public Security Bureau followed. See People's Daily and Beijing Youth Daily, 22 April 2005.
Wo chuan shàng yifú, kaiche dào gelúnbiya gaodì. Méiyou rén zài zhidìng de jiaoluò dengdài. Wo kaiche shiguò gai dìqu yiduàn shíjian, tíng xiàlái, zou huí jiaoluò. Zuìhòu, yi míng nuzi yuèguò gaosù gonglù yíngjie wo. Shì de, shì ta. Zhulì'an hé wo huí dàole wo de che. Wo gàosù ta, wo huì ba ta dài dào shèngbaoluó. Women kaishi cháo zhège fangxiàng xíngshi. Bùkesiyì de shì, zhulì'an ránhòu shuo, ta bùxiang qù shèngbaoluó. Ta ràng wo ba ta dài huí dào women jiànmiàn de jiaoluò li. Ta xià chele, yaoqiú $ 5.00. Wo gei ta qián
3K3A-APC also rescued the survival of iMNs from all 6 sporadic ALS lines (Figure 5H and Supplemental Figure 6, A–F; 6 sporadic ALS patients). When we tested iMNs from separate iPSC clones of the sporadic patient lines, 3K3A-APC also significantly improved their survival, indicating that the rescue effects were not specific to iMNs derived from certain iPSC clones (Supplemental Figure 6, G–J; 3 sporadic ALS patients). Reinforcing the notion that activating autophagosome formation in ALS iMNs could increase survival, rapamycin treatment significantly improved the survival of iMNs from 4 of 5 sporadic ALS lines tested (Supplemental Figure 7, A–J; 6 sporadic ALS patients). In contrast, rapamycin slightly decreased the survival of control iMNs (Supplemental Figure 7, K and L). These results suggest that rescuing autophagosome formation in sporadic ALS iMNs can increase their survival.
An intronic GGGGCC repeat expansion in C9ORF72 is the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), but the pathogenic mechanism of this repeat remains unclear. Using human induced motor neurons (iMNs), we found that repeat-expanded C9ORF72 was haploinsufficient in ALS. We found that C9ORF72 interacted with endosomes and was required for normal vesicle trafficking and lysosomal biogenesis in motor neurons. Repeat expansion reduced C9ORF72 expression, triggering neurodegeneration through two mechanisms: accumulation of glutamate receptors, leading to excitotoxicity, and impaired clearance of neurotoxic dipeptide repeat proteins derived from the repeat expansion. Thus, cooperativity between gain- and loss-of-function mechanisms led to neurodegeneration. Restoring C9ORF72 levels or augmenting its function with constitutively active RAB5 or chemical modulators of RAB5 effectors rescued patient neuron survival and ameliorated neurodegenerative processes in both gain- and loss-of-function C9ORF72 mouse models. Thus, modulating vesicle trafficking was able to rescue neurodegeneration caused by the C9ORF72 repeat expansion. Coupled with rare mutations in ALS2, FIG4, CHMP2B, OPTN and SQSTM1, our results reveal mechanistic convergence on vesicle trafficking in ALS and FTD.
Youle zhège jìshù bèijing, ràng women zhuanzhù yú “jiézòu hé zìwo yìshí” zhè ben shu de zhutí. Ta kenéng gèng shìdàng de cheng wèi “xíngshì, jiézòu hé zìwo yìshí”. Taolùn yousan gè bùfèn. Ànzhào chuántong zhéxué, xíngshì de taolùn fasheng zài dì èr hé dì san zhang. Jiézòu de taolùn, guanzhù yinyuè hé tiyù, zài dì sì, wu hé liù zhang. Zìwo yìshí de taolùn zài dì qi zhang, dì ba zhang, dì jiu zhang hé dì shí zhang zhong chongmanle zhè ben shu de qíyú bùfèn. Zìwo yìshí shèjí jiang zhùyì lì de jiaodian cóng shìsú shìjiàn zhuanyí dào guanchá zhe zìji de siwéi guòchéng.
The insistence of local journalists to maintain professional boundaries results from the ideology of professionalism that has permeated throughout China’s journalism education and practice since the reform and opening-up policy. Such insistence also manifests the responsive identification of these journalists under the context in which the journalistic profession is increasingly declining (Donsbach, 2010).

59 95% confidence interval ranged between 55 and 90%. Old Wang is only 33% likely to read commercialized papers (95% confidence ranged between 24 and 42%). A dummy variable for having travelled to countries in Europe or North America was dropped from the analysis, because it predicted the use of commercialized papers perfectly. Results can be retrieved from the author upon request.

Wang Xizhi ([wǎŋ ɕí.ʈʂɨ́]; Chinese: 王羲之; 303–361) is a Chinese calligrapher, writer and politician who lived during the Jin Dynasty (265–420), best known for his mastery of Chinese calligraphy. Wang is sometimes regarded as the greatest Chinese calligrapher in history, and was a master of all forms of Chinese calligraphy, especially the running script. Furthermore, he is known as one of the Four Talented Calligraphers (四賢) in Chinese calligraphy.[1] Emperor Taizong of Tang admired his works so much that the original Preface to the Poems Composed at the Orchid Pavilion (or Lanting Xu) was said to be buried with the emperor in his mausoleum.
Wo juédìng duì míngnísudá zhou shàngsù fayuàn tíchu shàngsù. Wo ye pìnqingle yi wèi xin de l?shi tángnàdé·xi er si tè lún (Donald Hillstrom) lái dàibiao wo. Women zuò chule yigè wúchi de huyù, daozhì fatíng tuifanle fà xi qí de juédìng. Ba zhège àn zi huán geile shenpàn faguan, zhishì ta bùdé bù xuanbù wo qizi céngjing maiguò fángzi de qián shì yi dàikuan háishì liwù de xíngshì chuxiàn.
Jíshi rúci, zhu lì an yeyou lánqiú. Zuòwéi yigè niánqing nuhái, ta bèi sòng dào xiàlìngyíng. Ha lái mu huánqiú luxíng zhe de qián chéngyuán zài yíngdì gongzuò rényuán. Ta xiàng niánqing de lùyíng zhe zhan shì liao rúhé ràng lánqiú gun xiàlái. Zhu lì an ài shàngle zhè xiàng yùndòng. Ta jingcháng zuò yigè niánqing nuhái. Zhè jiùshì wèishéme dang ta jìnrù àidísheng gaozhong de gao niánjí shí, ta xùnsù chéngwéi yi míng lánqiú míngxing. Ta sìhu duì yóuxì you tianfù.
iPSCs were tested for mycoplasma before, during, and after the study and were negative. iPSCs were first differentiated into fibroblast-like cells to enable efficient retroviral transduction. iPSCs (1 × 106 cells/flask) were seeded in a T75 flask that was coated with Matrigel (Corning) and cultured in mTeSR until reaching 70% to 80% confluence. Cells were then cultured in fibroblast medium (20% FBS in DMEM) for 14 days. Cells were passaged 1:1 with Accutase (Innovative Cell Technologies) to another flask that was coated with Matrigel and cultured in fibroblast medium until they reached 90% confluence. Cells were then passed with 0.25% trypsin to 3 flasks that were coated with 0.1% gelatin and cultured in 10% FBS in DMEM. Reprogramming of the fibroblast-like cells was performed in 96-well plates (8 × 103 cells/well) or 13-mm plastic coverslips (3.2 × 104 cells/coverslip) that had been sequentially coated with gelatin (0.1%, 1 hour) and laminin (2 to 4 hours) at room temperature. Seven iMN factors were added in 100 to 200 μL fibroblast medium per well of the 96-well plate with 5 mg/mL polybrene. Cultures were transduced with lentivirus encoding the Hb9::RFP reporter 48 hours after transduction with transcription factor–encoding retroviruses. On day 5, primary mouse cortical glial cells from P1 ICR pups were added to the transduced cultures in glia medium containing MEM (Life Technologies), 10% donor equine serum (HyClone), 20% glucose (Sigma-Aldrich), and 1% penicillin/streptomycin. On day 6, cultures were switched to N3 medium containing DMEM/F12 (Life Technologies), 2% FBS, 1% penicillin/streptomycin, N2 and B27 supplements (Life Technologies), 7.5 μM RepSox (Selleck), and 10 ng/mL each of GDNF, BDNF, and CNTF (R&D Systems). The iMN cultures were maintained in N3 medium, changed every other day, unless otherwise noted.
Understanding why most interviewed journalists also asserted that the assumptions that ‘multi-skilled journalists’ who can write, photograph and edit is unrealistic (if not entirely unreasonable), and claimed that newspaper journalists should be differentiated from specialised new media journalists who will transform into expert-type journalists in the future by delivering objective, rational and in-depth reportage on a certain subdivided domain. In this conception, new media practitioners remain quite distinct from their peers from the traditional media. However, such a difference has undergone slight changes, that is, traditional media practitioners have begun to admit and accept the possibility that their new media counterparts may be professionalised in the domain of news production. Journalists are consulting about their roles in reference to new media, although they are more inclined towards constriction rather than extension when adjusting their professional boundaries.
(A) Production of Hb9::RFP+ iMNs and survival tracking by time-lapse microscopy. (B and C) Survival of control (CTRL) and C9ORF72 ALS patient (C9-ALS) iMNs with a 12-hour pulse treatment of excess glutamate shown for each individual line separately (B), or for iMNs from all lines in aggregate (C). For B and C, n = 90 iMNs per line for 3 control and 2 C9-ALS lines, iMNs quantified from 3 biologically independent iMN conversions per line. (D) Survival of control and C9ORF72 ALS patient iMNs after withdrawal of neurotrophic factor supplementation. iMNs from all control or C9ORF72 patient lines shown in aggregate. n = 90 iMNs per line for 3 control and 3 C9-ALS lines, iMNs quantified from 3 biologically independent iMN conversions per line. (E and F) Survival of control and sporadic ALS (sALS) patient lines after glutamate treatment (E) or withdrawal of neurotrophic factor supplementation (F). iMNs from all control or C9ORF72 patient lines shown in aggregate. n = 90 iMNs per line for 3 control and 6 (E) or 5 (F) sporadic ALS lines, except sALS6 which had 60 (E) or 40 (F) iMNs counted. iMNs quantified from 3 biologically independent iMN conversions per line. (G–I) Immunofluorescence analysis of total TDP-43 (G) and quantification of the ratio of nuclear to cytoplasmic TDP-43 in control, C9-ALS (H), or sporadic ALS (I) iMNs. Ratio of nuclear to cytoplasmic TDP-43 in individual iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 6 days. iMNs from 2 controls and 2 C9-ALS patients (H) or 4 sporadic ALS patients (I) were quantified. n = 30 (controls), 30 (C9-ALS), or 36 (sporadic) iMNs per line per condition from 2 biologically independent iMN conversions of 2 control, 2 C9-ALS, or 4 sporadic ALS lines were quantified. Each gray circle represents a single iMN. Median ± interquartile range. Unpaired Mann-Whitney test. Scale bars: 5 μm. Dotted lines outline the nucleus and cell body. For iMN survival experiments, significance was measure by 2-sided log-rank test using the entire survival time course. The day of differentiation stated on each panel indicates the day of differentiation on which the experimental treatment or time course was initiated.
“Jiazu, zhongzu, guozu: Malaixiya huaren de yimin jingyu”  家族、种族、国族:马来西亚华人的移民境遇  (Family, Race, Nation: Malaysian Chinese Experience), presented at the International Conference on “Chinese Migration in Comparative Perspective: Adaptation and Development,”  华人移民比较研究:适应与发展  in Singapore, organized by the Centre for Chinese Language and Culture, Nanyang Technological University, and Chinese Heritage Centre, Singapore, 26-27 October 2007.

Yan, B., and M. Fang. 2009. Jiyu Web 2.0 de Shuzihua Jiaoxue Huanjing yu Ziyuan Jianshe de Yanjiu yu Shiyan (Research and Experiment on E-learning Environment and Resource Construction based on Web 2.0). Zhongguo Yuancheng Jiaoyu (Distance Education in China) (8): 32–40. [严冰, 方慕真. (2009). 基于 WEB2. 0 的数字化教学环境与资源建设的研究与实验. 《中国远程教育》, (8): 32–40.].Google Scholar
C9-ALS iMNs were cultured with 10 nM inactive 3K3A-APC or 3K3A-APC for 3 days and then Hb9::RFP+ iMNs were flow purified. 3′-Digital gene expression RNA-Seq of all samples was performed by Amaryllis Nucleics. Briefly, mRNA was extracted using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer’s instructions. 3′ RNA-Seq libraries were generated using the 3′-Digital Gene Expression RNAseq Library Kit (Amaryllis Nucleics). Libraries were sequenced on an Illumina NextSeq 500 sequencer. A total of 10 million to 25 million 80-bp, single-end reads were obtained for each sample. Reads were aligned to the Hg38 transcriptome using HISAT2 (41). A count table was obtained using FeatureCounts (42) with strand specificity enabled. Differential expression analysis was performed using DESeq2 (43). The DESeq2 sample description table used, count table, and DESeq2 results can be found in the supplemental data. The sequencing data have been deposited in NCBI’s Gene Expression Omnibus (GEO) and can be accessed using GEO Series accession number GSE133162.
Ji gè xingqí yilái, xìng jinzhang kaishile. Lín dá shuì zài yi jiàn wòshì li, wo zài lìng yi jiàn wòshì. Wo zunzhòng ta dì xìng quxiàng. Jíshi rúci, yidàn ta zhàn zài wo de fángjian li, chuanzhuó hànshan, wo túrán ba kùzi yizhí la xiàlái. Lín dá sìhu hen xingfèn ji tianhòu, dang wo zuò zài wo de bàngong zhuo qián, wèn wo shìfou you anquántào. Wo méiyou. Bùguò, women hen kuài jiù tuo xià yifú, zài wo de chuángshàng zuò'ài. Nà shì yigè zhang dá yigè xingqí dì xìng guanxì de kaishi. Lín dá yaoqiú kekào de biaoxiàn. Women zài xuduo fangmiàn dou hen qinmì.

Such a characteristic of we-media has further squeezed the price bidding space of the new media of Fujian press industry in the area of content in local journalists’ eyes. What is worse, the procedure of three-phase censorship that deviates from the logic of new media is not strictly followed during the course of converging news production. Delays in the course of censorship have not only impaired the market competitiveness of the new media in terms of content, but also considerably reduced the willingness of journalists to collaborate with the new media centre.

Ránhòu zài 1454 nián, gu deng bó gé famíng liao ke yídòng yìnshua, zhè yi xìliè de dì san gè shìjiàn. Zhongguó hé hánguó zài ouzhou rén zhiqián kaifale yìnshua jìshù; ta zài táng cháo qíjian yòng yú dàliàng shengchan fójiào wénxué. Rán'ér, yuandong de jiaoben, fei zìmu, bùshìyìng yìnshua bi ouzhou jiaoben, suoyi zhè zhong jìshù duì tamen de wénhuà yingxiang jiào xiao.
Approaches to writing instruction developed in North America have gradually made their presence felt in other parts of the world during recent years. A curricular evaluation of the local needs, instruction, assessments, teacher preparation, and other pedagogical factors is crucial for the successful transmission and integration of those approaches into the new contexts. Set against the background of recent, exuberant scholarly discussions of the issue of transplanting Western writing pedagogies, this article presents an observational report of a typical college English curriculum for non-majors in China, with a focus on its writing component. The study has found that English writing is taught under the guidance of a nationally unified syllabus and examination system. Rather than assisting their students to develop thoughts in writing, teachers in this system are predominantly concerned about the teaching of correct form and test-taking skills. Because of their relatively low economic status in China, English teachers have to work extra hours and have little time to spend on individual students or on furthering their professional training. However, signs of recent Western writing pedagogies, such as pre-writing and multiple-drafting activities, are identified in classrooms and textbook publishing, which indicate the possibility of successful adaptations of the recent Western writing pedagogies in the Chinese context.
Wang Xizhi is particularly remembered for one of his hobbies, that of rearing geese. Legend has it that he learned that the key to how to turn his wrist whilst writing was to observe how geese moved their necks. There is a small porcelain cup depicting Wang Xizhi "walking geese" in the China Gallery of the Asian Civilisations Museum in Singapore. The other side of the cup depicts a scholar "taking a zither to a friend".
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Lián jingcháng gàosù wo ta bù kaixin. Wo shì yigè hen zìsi de rén, hen shao guanzhù qizi de xuyào. Dang ta yaoqiú yi liàng che shí, wo jùjué maile yi liàng xinche. Shenti bù hao, ta jìxù chouyan. Ta zuò zài ta danwèi de kèting li, kànzhe zhongwén lùyindài huò zài gèrén diànnao shàng wánle zhipái. Jinnián wan xie shíhòu, women cóng shèngdìyàge dào xià jialìfúníya zhou de ka bo shèng lú ka si jìnxíngle wéiqí yizhou de xúnháng.
Zài zhongguó zhi xíng zhong, wo céng wènguò youyi míng wèihun mèimei de daoyóu, shìfou you kenéng huì yùjiàn yi míng zhongguó nuzi wéi hunyin mùdì. Zhè zhishì yigè cháxún. Dangrán, wo háishì hé xila jiéhun, dàn wèntí zàiyú women de guanxì fazhan. Zhongguó daoyóu hòulái chéngwéi tiánnàxi dàxué zài chá ta nu jia de xuésheng. Zài shenghuó zài zhège chéngshì de shíhòu, ta zài yigè shèjiao jùhuì shàng yù dàole yigè zhongguó nurén, ta shuo jiejie you xìngqù jià gei yigè meiguó rén. Zhè jiùshì wo yù dàole wo de dì san rèn qizi. Daoyóu da diànhuà gei wo, hé wo liánxì de jiejie, ta xiàng wo fasòngle yigè hen kuài chéngwéi wo qizi de nurén de liánxì fangshì. Zài yóujiàn liánxì bùjiu zhihòu, wo anpái zài beijing bàifang ta.
Liánxù 10 yuè 18 rì xingqí yi, wo hé wo du fei huíle míng ní abo lì si. Women cóng tongcháng dì lùndian kaishi. Hòulái, dang wo hé wò'erma yiqi qù gòuwù, ránhòu zài chaojí dà zìzhùcan zhong chi wufàn shí, women de guanxì dédào gaishàn. Liánxiang yào wo gèng duo de guanzhù ta. Yiqi qí che shì jijí de jinglì. Ta yú 11 yuè 2 rì xingqí yi huí dào zhongguó, zhè shì dàxuan dàngtian.
Shumiàn yuyán cóng shangyè kaishi. Gudài su mei er de shangrén xuyào yi zhong fangfa lái genzong shangyè jiaoyì. Rúguo shangrén chúcún guwù, ta xuyào zhidào zài tèdìng wèizhì baocún mei zhong lèixíng guwù de duoshao. Lìshi xué jia rènwéi, su mei er shangrén shiyòng yóu kao niántu zhì chéng de dài bì lái xiàngzheng dàliàng de shangpin. Yi zhong xíngzhuàng kenéng yìwèizhe liù púshì'er xiaomài; lìng yigè, yi púshì'er xiaomài; hái youyi jialún ganlan yóu. Tongguò jiang zhèyàng de biaojì fàngzhì zài wan xíng niántu róngqì zhong bìngqie xiangyìng dì biaojì wàibù, shangjia jiang zhidào tasuo yongyou de mei zhong shangpin de duoshao hé duoshao. Dàng shangrén yìshí dào wan limiàn de niántu biaojì suo dàibiao de xiangtóng xìnxi yóu wàimiàn de biaojì biaodá shí, zhèngquè de xiezuò kaishi. Yinci, tamen keyi mian qù wan hé yi píngban diànnao shàng míngwén de xíngshì tígong xìnxi.
According to Mai (2012: 155–168), these two paths have rendered the cooperative interactions between the traditional and new media outlets far less frequent, if not totally suspended, and are likely to further alienate the newspapers from the original or latest new media departments. In other words, both paths create new administrative barriers whilst breaching or circumventing the original administrative barriers.
Wo de shourù zhifùle women hunyin qíjian de dà bùfèn jiatíng zhichu, ér wo de qizi de shourù shì cháozhe zhubao huò ta xiang yào de rènhé qíta de dongxi. Wo muqin mai de yixie you jiàzhí de danmài shèngdàn sháo chuláile. Wo de qizi shouxian shuo ta bù zhidào sháozi zài nali, dànshì dang wo tíjiao baoxian sunshi suopéi shí, ta shuo tamen shì zài “cúnchú”. Shougòu cáichan sìhu shì ta duì zhè xiàng hunyin de zhuyào xìngqù.
6 J.J. Kennedy, “Maintaining popular support for the Chinese Communist Party: the influence of education and the state-controlled media,” Political Studies, Vol. 57, No. 3 (2009), pp. 517–36; Chen, X. and Shi, T., “Media effects on political confidence and trust in the People's Republic of China in the post-Tiananmen period,” East Asia: An International Quarterly, Vol. 19, No. 3 (2001), pp. 84–118.

To determine how 3K3A-APC might stimulate autophagosome formation, we performed RNA sequencing (RNA-Seq) on C9ORF72 ALS iMNs treated with 3K3A-APC or inactive 3K3A-APC for 3 days (2 C9ORF72 ALS patients). Consistent with our findings in the mRFP-GFP-LC3 and immunoblot assays, both Ingenuity Pathway Analysis (IPA) and KEGG pathway analysis revealed “autophagy” as one of the most significantly enriched gene sets (Supplemental Figure 2, E–H). RNA-Seq and quantitative real-time PCR (qRT-PCR) showed that 3K3A-APC significantly increased the expression of ATG5 and ATG10, which encode proteins with key roles in driving autophagosome formation (ref. 25, Figure 2D [qRT-PCR], Supplemental Figure 2I [RNA-Seq], and Supplemental Table 4). Thus, 3K3A-APC increases the expression of genes encoding autophagy-initiating factors in ALS iMNs.
Wo 11 yuè 24 rì fei wang zhongguó, huale shí tian shíjian yu lián. Women jìhuà qù yuènán luxíng, dànshì yóuyú wo méiyou zhòng wén de wénjiàn, suoyi méiyou dédào jiejué. Women ye xiwàng qù tianjin yitian de luxíng, dàn méiyou. Women huale zhengzheng yigè shíjian zài beijing, zài nàli maile xin yanjìng, bìng pèile yìchi. Wo yigè rén huí dào míngnísudá zhou,12 yuè 5 rì língchén dàodá.
“Detraditionalized and Renewed Qiaoxiang Areas: Case Studies of Panyu and Wenzhou in the Reform Period since 1978,” presented at the 5th Conference of the International Society of the Study of Chinese Overseas “New Chinese Migrants – Globalization of Chinese Overseas Migration,” in Elsinore, Denmark, organized by the Nordic Institute of Asian Studies and the Department of Asian Studies, University of Copenhagen, 10-14 May 2004.

Zài bólatú, su géla di de méntú hé zhuànjì zuòzhe shouzhong, zhèxie lixiang bùjin jin shì xíngwéi lèibié. Tamen benshen shì youqù de, zuòwéi yi zhong xin de cúnzài de lìzi. Zhèxie lixiang shì cíyu, huòzhe gèng zhunquè de shuo, shì zhàn zài cí hòumiàn de chúncuì de lèixíng. Tamen shì gè zhong gè yàng de pubiàn xìng. Pubiàn xìng de gàiniàn zài rénlèi wénhuà de zhè yidian shàng shì xin de.
Xi lì yà zài shèng ào lafu xiangshòuguò ta de suìyuè. Jian ér yán zhi, ta you yigè zhongguó nán péngyou gei ta yi zhi húlí gou de xiao gou zuòwéi shengrì liwù. Wèile wo de kuàilè, gou zài wo de guanxin zhong jiéshùle. Liánméng bù zànchéng nán péngyou, yinwèi ta ye duì qíta nurén shuo. Ta hé xi lì yà hen kuài jiù fenshoule. Jù wo suo zhi, ta hái méiyou rènzhen duìdài rènhé rén.
Zài lìshi de zhège jieduàn, shèhuì canyù chuàngzào dàxíng zhèngzhì dìguó. Xuyào shujì yuán chuli nèibù goutong yi guanli guangdà de lingtu. Xiezuò de yìshù yu zhèngfu guanliáo jigòu de xíngchéng mì bùkefen. Rénmen rènwéi zài yilakè nánbù jiànzào aijí jinzìta huò wéichí yùnhé xuyào dà guimó de nulì. Zài dà duoshù wénmáng shèhuì zhong, zhiyào yourén yongyou jiànlì dàxíng jìniànbei huò zhèngquè bèisòng qídao de jìshù zhishì jiù zúgòule; yixie shu jiàng keyi cóng shumiàn jiansuo.
A doxycycline-inducible (Dox-inducible) NGN2, ISL1, LHX3 (NIL) polycistronic construct was previously integrated into the AAVS1 safe harbor locus of the C9-ALS patient iPSC line using CRISPR/Cas9 editing (4). Dox-NIL iMNs were generated by plating at approximately 25% confluence on Matrigel-coated plates and adding 1 mg/mL Dox in N3 media plus 7.5 μM RepSox 1 day after plating. Mouse primary mixed glia were added to the cultures on day 6, and Dox was maintained throughout conversion. iMN cultures were harvested on day 26.
5 Yuè 25 rì zuoyòu, míng ní abo lì si shì zhang hòuxuan rén bào bó·ka ní (Bob Carney) da diànhuà xúnwèn wo shìfou huì hé ta yiqi churèn míngnísudá zhou fù zhouzhang. Ta zhèngzài gònghédang xiaoxuésheng zhong danrèn “wenhé, jìnqu de gònghédang rén”. Bào bó xiàng wo baozhèng, wo bùbì zuò rènhé gongzuò. Shenqing fèi shì $ 300, dàn ta huì wèi wo zhifù. Wo tíchu zhifù 200 meiyuán de fèiyòng. Zhè shì wo keyi chuli de shìqíng - ràng biérén dàitóu canyù zhèngzhì yùndòng. Zài 2010 nián 9 yuè 10 rì juxíng de 2010 nián gònghédang zhuxí xiaozu zhong, bào bó hé wo pái zài sì míng hòuxuan rén zhong de dì èr míng. Yu tangmu·ài mò màn de 107,600 piào xiang bi, women yongyou yue 9,800 piào huò 7.5%De piàoshù.
Dang wo hái zhù zài báixióng hú de xiaowu shí, wo jìlùle yigè míng jiào ma sha de nurén. 1983 Nián, zài míngnísudá beibù de zhènwáng jiàngshì jìniàn rì zhoumò, wo yù dàole yigè dú mùzhou de luxíng dào míngnísudá zhou beibù de bianjiè shuiyù. Wo de péngyou ha wéi·kai yuè (Harvey Hyatt) shì zhè cì luxíng de zuzhi zhe. Ma sha dangshí shì “míngnísudá zhou xiàngmù” de zhíxíng dongshì, zhè shì yóu xiànrèn míngnísudá zhou zhouzhang makè·dài dùn (Mark Dayton) kaishi de fei yínglì zuzhi. Ta hé wo zài zhè cì luxíng zhong dale gè dun, women yigè xingqí hòu kaishi yuehuì. Ta zhèng shìtú pínghéng ta de zhìlì zìwo (zuòwéi yigè guanli zhe) yu ta de nèixin huò qínggan de zìwo. Genjù ta de jiànyì, liù yuè geshou dúle “línghún de jièxiàn: Róng gé xinli xué de shíjiàn”.

Taking advantage of Fuchai's expedition to his north to defeat Qi, Goujian led his army and successfully attacked the Wu capital, killing the Wu crown prince, You. In the 24th year of his reign (473 BC), Goujian led another expedition against Wu, laying siege to the capital for three years before it fell. When a surrender from Fuchai was refused, Fuchai committed suicide and Wu was annexed by Yue. After his victory, Goujian ruthlessly killed Fuchai's scholars, even those who helped him (including Bo Pi), not allowing himself to make the same mistake Fuchai had made by sparing the lives of his enemies. However, Goujian would not stop there; he would later force Wen Zhong to commit suicide; Fan Li, knowing that Goujian was a man who can share woe but not wealth together, left Goujian after the defeat of Wu.
16 Expertise is defined as a source's “presumed knowledge and ability to provide accurate information.” See Petty, R. and Wegener, D., Attitude Change: Multiple Roles for Attitude Change (Boston: McGraw-Hill, 1998), p. 344. Objectivity refers to perceptions of media sources to be unbiased, accurate, fair and “to tell the whole story.” See Iyengar, S. and Kinder, D.R., Psychological Accounts of Agenda-Setting (Beverly Hills: Sage, 1985); Miller, J. and Krosnick, J., “News media impact on the ingredients of presidential evaluations: politically knowledgeable citizens are guided by a trusted source,” American Journal of Political Science, Vol. 44, No. 2 (2000), pp. 301–15.
Bùjiu zhihòu, xila jiù zhìliáo kekayin yilài. Ta fùfa yicì, ránhòu jìnrù lìng yigè zhìliáo fang'àn. Ta zhù zài Anoka de yigè zhongtú de fángzi yiduàn shíjian. Dang wo fangwèn shí, women youshí zài shì zhongxin de kafei diàn wán qí. Xila jingcháng ting jidujiào guangbò de línggan, ràng ta zou shàng fùsu de dàolù. Ta jíshí ba ta de háizi dài huílái, chéngwéi yigè rènzhen de muqin.
Although the competitiveness of commercial new media are discerned, the interviewed journalists remain inclined to regard the contents of new media as ‘superficial’, ‘fast food-like’ and ‘targeting lower-level readers’. Accordingly, the journalists believe that the poor quality of the content provided by the nonprofessional new media outlets further highlights the importance of the journalists’ professional expertise of separating falsity from truth.
Quantification of the effect of TMX2 reduction by shRNA lentiviral transduction in two independent control iPSC lines, as performed in Fig. 7. Induced motor neurons were generated from iPSCs using a seven factor (7F) differentiation system. The survival of HB9-RFP+/shRNA-GFP+ iMNs was tracked by imaging after the addition of 10 µM glutamate to the cultures. All iMN survival experiments were analyzed by log-rank test, and statistical significance was calculated using the entire survival time course. *p < 0.05, ** p < 0.01 (survival assays were performed in triplicate with the indicated number of iMNs analyzed for each group, Supplementary Table 4).
Liányì huì zài 2003 nián shèngdàn jié qíjian shenqingle wèiyú míng ní abo lì si shì zhongxin de Target shangdiàn de línshí zhíwèi. Yóuyú ta de gongzuò liánghao, ta bèi yaoqiú zuòwéi yi míng yongjiu xìng gùyuán liú xià. Ta de gongzuò rènwù shì qingxiàng yú zhuangxiu shì. Zhège zhíwèi di yú ta zài zhongguó de zhíwèi, dànshì ta hé ta de jingli rén hé tóngshì youzhe xióngxin bóbó de guanxì. Duìyú wo lái shuo, wo hen gaoxìng kàn dào wo de qizi zhàn zài ta hóng liàng de hóngsè zhìfú dì dìban shàng. Dang ta zài 2005 nián bèi píng wèi “weidà de tuánduì yingxióng” shí, wo xiele yi shou shi.
3K3A-APC was produced as described previously (23) and was a gift from ZZ Biotech. Human 3K3A-Protein C (3K3A-PC) zymogen was stably transfected into Chinese hamster ovary (CHO) cells that were grown in suspension in CD OptiCHO medium (Invitrogen) supplemented with 2 mM CaCl2, 10 μg/mL vitamin K, and 2 mM GlutaMAX (Invitrogen). After the required number of serial expansions of culture volumes using shake flask passages and WAVE culturing, the final stirred-tank bioreactor volume was either 200 L or 2500 L in which culture was performed for 10 days at 35°C. After 0.2-μm filtration, the clarified culture supernatant was purified using chromatography on a Q Sepharose fast-flow ion-exchange column and hydrophobic affinity column. The purified 3K3A-PC zymogen was then activated with recombinant human thrombin (Recothrom, Zymogenetics) to generate 3K3A-APC. Thrombin was removed from the reaction mixture using ion-exchange chromatography with a UNOsphere S flow-through resin (Bio-Rad). The concentration of 3K3A-APC was adjusted using tangential flow filtration. Following the concentration step, UNOsphere Q ion-exchange flow through was used with buffer exchange and concentration adjustment, prior to Planova filtration and addition of polysorbate-80. 3K3A-APC was characterized by SDS-PAGE under reducing and nonreducing conditions, SE-FPLC, amidolytic activity by a chromogenic assay, and optical density at 280 nm. Inactive 3K3A-APC was generated by heat-denaturing APC.
Taking advantage of Fuchai's expedition to his north to defeat Qi, Goujian led his army and successfully attacked the Wu capital, killing the Wu crown prince, You. In the 24th year of his reign (473 BC), Goujian led another expedition against Wu, laying siege to the capital for three years before it fell. When a surrender from Fuchai was refused, Fuchai committed suicide and Wu was annexed by Yue. After his victory, Goujian ruthlessly killed Fuchai's scholars, even those who helped him (including Bo Pi), not allowing himself to make the same mistake Fuchai had made by sparing the lives of his enemies. However, Goujian would not stop there; he would later force Wen Zhong to commit suicide; Fan Li, knowing that Goujian was a man who can share woe but not wealth together, left Goujian after the defeat of Wu.
Sep 2018: Our first IEEE TKDE paper has been accepted! The paper ‘‘Efficient and Distributed Generalized Canonical Correlations Analysis for Big Multiview Data’’ comes from a collaborative work with CMU (Prof. Christos Faloutsos and Prof. Tom Mitchell). Now the team members are spread across the U.S. and the world (OSU,UFL,CMU,UVA,UCR,IIS). Congratualations to all! The full paper will be uploaded soon.

Xila hòulái jieshì shuo, ta céngjing zài beijiao zhensuo de yi wèi jingshénbìng xué gùwèn gàosù wo, wo de dì dì an dí, zài diànshì li hé háizimen yiqi kàn kongbù piàn hòu, céngjing shuoguò, zhèxie háizi “xiang yào bèi zhège guài tai nánzi qiángjian”. an dí, cubào de hé chaozhòng, kenéng kàn qilái xiàng yigè bogeyman. Yinci, xinli xué jia dé chu jiélùn, wo gege rènwéi xila de háizi xiang yào bèi ta xìngsaorao. an dí xihuan háizi, dàn ta yongyuan bù huì hong tamen. Zhè shì yigè bùxìng de xuanzé. Bùguò, fudao yuán shuo, rúguo Andy jìxù hé Sheila de háizi zhù zài tóngyi suo fángzi li, ta jiang bùdé bù xiàng értóng baohù shìjiàn huìbào shìjiàn. Wo bù huì ba wo de xiongdì ti chu wojia. Suoyi, xila juédìng banjia.
King Goujian's army was known for scaring its enemies before battle because its front line consisted of criminals sentenced to death who committed suicide by decapitating themselves.[2] However, in the passage, "越王句踐使死士挑戰,三行,至吳陳,呼而自剄。", the literal translation of "死士" is "soldiers (who are) willing to die", not "criminals sentenced to death". "自剄" means to "commit suicide by cutting one's throat," which was a common way to end one's own life in Ancient China.[3]
Understanding why most interviewed journalists also asserted that the assumptions that ‘multi-skilled journalists’ who can write, photograph and edit is unrealistic (if not entirely unreasonable), and claimed that newspaper journalists should be differentiated from specialised new media journalists who will transform into expert-type journalists in the future by delivering objective, rational and in-depth reportage on a certain subdivided domain. In this conception, new media practitioners remain quite distinct from their peers from the traditional media. However, such a difference has undergone slight changes, that is, traditional media practitioners have begun to admit and accept the possibility that their new media counterparts may be professionalised in the domain of news production. Journalists are consulting about their roles in reference to new media, although they are more inclined towards constriction rather than extension when adjusting their professional boundaries.
May 2018: Prof. Nikos Sidiropoulos (ECE at University of Virginia) gave a Keynote at ICC Workshop ‘‘Machine Learning for Communications’’ in Kansas City. See the slides here. Interesting recent results of using deep neural networks to solve a wireless resource allocation (power allocation) problem are included in the slides (with details in see details in this paper ‘‘Learning to Optimize: Training Deep Neural Networks for Wireless Resource Management’’).
“Jiaoyu ziben yu guojia xitong: Nanda de zuihou wu nian”  教育资本与国家系统:南大的最后五年  (Education Capital and National System: The Last Five Years of Nanyang University), presented at Nantah History Workship on “Zuqun, jiaoyu, guojia: 1953 zhi 1980 nian de Nanyang Daxue”  族群、教育、国家:1953至1980年的南洋大学 (Ethnicity, Education, and Country: Nanyang University, 1953-1980), in Singapore organized by the Centre for Chinese Language & Culture, Nanyang Technological University, 5 August 2006.
Faguan zuòle 6 gè yuè. Ránhòu women zàicì chutíng. Wèile sheng qián, wo dàibiaole zìji. Lìng yi wèi l?shi shìtú zài chutíng miànqián yu wo shuohuà, dàn wo jianchí tingzhèng huì jìnxíng. Faguan zàicì méiyou qingli gongpíng de cáichan fenge. Ta jùjué zuò shàngsù fayuàn de mìnglìng: Shuomíng wo muqin hé xiongdì de qián shìfou shì dàikuan huò liwù. Ta qicaoliao lìng yi fèn wénjiàn, wénzì lüè you bùtóng, dàn shízhì què xiangtóng.
Nevertheless, the resistance from journalists makes no difference to the convergence path of China’s local press industry. The tendency for senior journalists to be more devoted to converging news production compared with junior ones and the tendency for journalists from party organs to show a higher positivity towards contributing compared with those from metropolis newspapers both highlight the impact of institutional and organisational factors on the journalists’ intention and behaviour in collaboration.

 Zài rènhé changsuo, guanzhòng xiang yào yanzòu shanshuò de jiézòu. Tamen xiang yào ganxìng de biaoyan - dàdan de zhanlan, yiqiè sìhu dou zhèngquè - tamen zànshang keyi tígong zhè zhong tíshì de biaoyan zhe. Tamen pèifú yùndòngyuán, jinguan chongtú de yalì, keyi yíngdé guànjunsài; huò keyi zài baozhuang de tiyùchang nèi jifa guanzhòng de liúxíng geshou; huò xijù yanyuán zài diànshì jiémù zhong de zhèngquè jiechù. Zài rènhé biaoyan fangshì, gongzhòng jìngpèi biaoyan zhe shuí keyi ràng zìji de changhé, bìng gei chu yigè hao de biaoyan, dang ta de zhòngyào.
As part of a political project to seize ‘the commanding heights’ of information dissemination, namely to strengthen the traditional press’s influence in shaping public opinion, practices of media convergence within Fujian’s press groups are substantively compliant with the ‘top-down design’ that is aimed to effectively circumvent political risks and maximise fiscal investment and market return. To bypass the political minefield and manage other risks that the new media centre may encounter whilst promoting convergence, Fujian’s press groups built a three-phase censorship system of ‘editor–new media centre director–press leadership’, among many other measures. Rigorous controls over convergence risks have enabled the Fujian press industry to gain the anticipated policy bonus. For example, the new media centre, for which the Xiamen Daily Group invested over one million Yuan, significantly affected municipal leaders, thereby earning over 20 million Yuan of financial subsidies for the centre in 2015.

Dang alán da diànhuà gàosù a shén lì da diànhuà gei mìxixibi zhou de shíhòu, wo yijing anpáile yigè chu wù guì, wèn ta de bànlu zhulì'an shìfou keyi ba ta de yixie wùpin ná zou. Dangrán, wo tóngyìle. Zhulì'an shanchú de yiqiè dou shì wo bùbì qù cúnchú de dongxi. Suoyi wo ràng ta huí dào gongyù li ba Ashley de yifú fàng zài dàizi li. Dang ta líkai dàizi de shíhòu, ta wèn ta shìfou keyi zài yitian nèi huílái zài ná yixie wùpin. Wo ye tóngyìle.
(A) mRFP-GFP-LC3 fluorescence in control, C9-ALS, or sporadic ALS (sALS) iMNs treated with or without 50 nM bafilomycin and 10 nM inactive 3K3A-APC or 3K3A-APC. Scale bars: 5 μm. Solid and dotted lines outline the cell body and nucleus, respectively. Cell bodies were visualized with mRFP-GFP-LC3 fluorescence using a longer exposure and increased gain. (B and C) Number of RFP+GFP+ vesicles per iMN in control, C9-ALS (B), or sporadic ALS (C) iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC and 50 nM bafilomycin for 24 hours. iMNs from 3 controls, 3 C9-ALS (B), and 5 sporadic ALS (C) patients were quantified. n = 12 iMNs per line per condition across 2 independent iMN conversions were quantified. Each gray circle represents a single iMN. Median ± interquartile range. Kruskal-Wallis testing. (D) qRT-PCR analysis of mRNA levels of ATG5 and ATG10 in C9-ALS iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 3 days. n = 4 independent iMN conversions and treatments per condition. Each gray circle represents a single RNA sample. Mean ± SD. Two-tailed t test, unpaired. (E) Number of LAMP2+ vesicles in control or C9-ALS iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 24 hours. iMNs from 2 controls and 2 C9-ALS patients were quantified. n = 21 iMNs per line per condition across 2 independent iMN conversions were quantified. Each gray circle represents a single iMN. Mean ± SEM. One-way ANOVA. (F) Number of LAMP2+ vesicles in control or sporadic ALS iMNs treated with 10 nM inactive 3K3A-APC or 3K3A-APC for 24 hours. Each gray circle represents a single iMN. iMNs from 2 controls and 6 sporadic ALS patients were quantified. n = 33 iMNs per line per condition across 2 independent iMN conversions were quantified. Median ± interquartile range. Kruskal-Wallis testing. The day of differentiation stated on each panel indicates the day of differentiation on which the experimental treatment or time course was initiated.
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