C9ORF72 ALS iMNs have fewer lysosomes than control iMNs (4), which could affect autophagic flux downstream of autophagosome formation. To determine if 3K3A-APC affects lysosome number in C9ORF72 ALS iMNs, we examined the number of LAMP2+ vesicles in iMNs (4). 3K3A-APC restored normal lysosome numbers in C9ORF72 ALS iMNs (Figure 2E; 2 controls and 2 C9ORF72 ALS patients, and Supplemental Figure 2, L and M). 3K3A-APC did not alter C9ORF72 protein (Supplemental Figure 2, N and O; 3 C9ORF72 ALS patients) or mRNA levels (Supplemental Figure 2P) in C9ORF72 ALS iMNs. Therefore, 3K3A-APC rescues lysosome numbers in C9ORF72 ALS iMNs without altering C9ORF72 levels.
Mice were anesthetized with i.p. ketamine (100 mg/kg) and xylazine (10 mg/kg), and body temperature kept at 36.9°C ± 0.1°C with a thermostatic heating pad. Mice were placed in a stereotactic apparatus (ASI Instruments) and the head was fixed accordingly. A burr hole was drilled, and an injection needle (33 gauge) was lowered into the hippocampus between CA1 and the dentate gyrus (anteroposterior −2.0, mediolateral +1.5, dorsoventral −1.8). NMDA (10 nmoles) and 0.2 μg of 3K3A-APC or vehicle in 0.3 μL of PBS, pH 7.4 was infused over 2 minutes using a micro-injection system (World Precision Instruments).

Generally, the journalists’ reluctance to collaborate can be explained under the binary structure of ‘traditional media versus new media’, which is manifested at three levels. At the institutional level, the antithesis of traditional media versus new media is represented as the schism between ‘inside the system’ and ‘outside the system’. Given the impact of new media and the existing ideological control, the Fujian press industry has opted to sacrifice part of its economic gains for political security, which is also an option for journalists ‘inside the system’. At the organisational level, the antithesis is manifested as the enmity between the traditional and the new media departments. Divergent interests have made the leaders and journalists of metropolis newspapers less willing to cooperate with the centre compared with their counterparts from the party organs. At the individual level, the binary structure is manifested as the competition between professional journalists and other we-media runners. Faced with challenges from non-professional information providers, most journalists opt to follow their occupational boundaries and refrain from adding their voice to the new media.
Wénlín yì wéi “qúnyīng huì” yǔ gōngsī míng xiāngtóng de shì, Wénlín Yánjiūsuǒ de chéngxùyuán dàitóurén dōu shì shēnjiān Hànyǔ yǔyánxuéjiā hé jìsuànjī kēxué gōngchéngshī liǎng zhǒng shēnfèn. Guānchá děngshì liǎngbiān, zhè ràng Wénlín Yánjiūsuǒ de chuàngshǐrén hé zǒngcái Tuōmǎsī E. Bìxiǎopǔ (Thomas E. Bishop) chóngxīn fāxiàn le Hànyǔ shénmì de 36 bǐ “zìmǔ”, jìn'ér yīláoyǒngyì de jiějué le Unicode “quēzì” de wèntí. Zhèxiē chuántǒng jiǎoběn yuánsù yīzhí yǐlái dōu bèi ruǎnjiàn kāifāzhě suǒ hūshì. “CDL yīzhí shì Wénlín de yī bùfèn, dàn wǒmen wèi fāxiàn qiánzài yǔyán, zhídào Wénlín 4.0 bǎn tuīchū. Rújīn yònghù kěyǐ chákàn bìng cāokòng rènhé kě zài bǐhuà kuàng zhōng chákàn de zìfú de CDL miáoshù,” Bìxiǎopǔ shuōdào. Wénlín Yánjiūsuǒ shì Unicode Xiéhuì de róngyù huìyuán, zì 2000 nián yǐlái yīzhí zhìlìyú míhé CJK shùjù chǔlǐ shang de chājù.
“Guxiang, guojia, shichang: Xin Ma he Xianggang Panyu huiguan de gongneng yanbian” 故乡,国家,市场:新马和香港番禺会馆的功能演变  (Homeland, State, and Market: The Changing Functions of Panyu Associations in Singapore, Malaysia, and Hong Kong) in Liao Chiyang 廖赤阳and Liu Hong  刘宏 (eds.), Cuozong yu shichang, shehui yu guojia zhijian: Dongya kouan chengshi de huashang yu Yazhou quyu wangluo 错综与市场、社会与国家之间:东亚口岸城市的华商与亚洲区域网络  (Surfing the Interfaces of Market, Society and the States: Chinese Merchants in East Asian Coastal Cities and the Making of Business Networks).  Singapore: Centre for Chinese Language & Culture, Nanyang Technological University, and Global Publishing, 2008, pp. 189-210.

In the early 17th century, persistent drought and famine driven by the Little Ice Age accelerated the collapse of the Ming dynasty.[9] Two major popular uprisings swelled up, led by Zhang Xianzhong and Li Zicheng, both poor men from famine-hit Shaanxi who took up arms in the 1620s.[10] At the same time, Ming armies were occupied in the defence of the northern border against the Manchu ruler Hong Taiji, whose father, Nurhaci, had united the Manchu tribes into a cohesive force. In 1636, after years of campaigns against Ming fortifications north of the Great Wall, Huangtaiji declared himself emperor of the Qing dynasty.[11]
We thank the NINDS Biorepository at Coriell Institute for providing the cell lines used for this study. Whole-genome sequencing was provided by the University of Washington Center for Mendelian Genomics (UW-CMG) and was funded by NHGRI and NHLBI grants UM1 HG006493 and U24 HG008956 and by the Office of the Director, NIH, under award number S10OD021553. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. We thank Emily Wong and Anton Valouev for assistance with whole-genome sequencing and analysis. We thank Helen Falk and Sandy Falk of Ji Informatics for performing RNA-Seq analysis. We thank USC Libraries Bioinformatics Service for assisting with data analysis. We thank the Choi Family Therapeutic Screening Facility for chemical screening support and DRVision LLC for imaging support. 3K3A-APC was a gift from ZZ Biotech. This work was supported by in part by NIH grant NS090904 to BVZ and startup funds to BVZ. This work was also supported by NIH grants R00NS077435 and R01NS097850, US Department of Defense grant W81XWH-15-1-0187, and grants from the Donald E. and Delia B. Baxter Foundation, the Tau Consortium, the Frick Foundation for ALS Research, the Muscular Dystrophy Association, the New York Stem Cell Foundation, the Alzheimer′s Drug Discovery Foundation, the Association for Frontotemporal Degeneration, the Pape Adams Foundation, the John Douglas French Alzheimer′s Foundation, the Harrington Discovery Institute, the Merkin Family Foundation, the USC Keck School of Medicine Regenerative Medicine Initiative, the USC Broad Innovation Award, the University of Southern California Alzheimer′s Disease Research Center, and the Southern California Clinical and Translational Science Institute to JKI. JKI is a New York Stem Cell Foundation-Robertson Investigator. KAS was supported in part by a Muscular Dystrophy Association development grant.
Dangshí wo hái you qíta de tieqì zài huo zhong. Zài 2001 nián 9 yuè 11 rì, zài míng ní abo lì si shì zhang de zhuyào zhànbài zhong, wo jiarùle míngnísudá zhou dúlì dang. 2002 Nián 7 yuè, wo xiàng xiaozu weiyuánhuì dì meiguó canyìyuàn hòuxuan rén tíchu zhíyí. Zhège yùndòng zhuyào shi wéiràozhe guójia fangwèn bàoshè. Jiéguo shì zài san rén bisài zhong zhàn 31%de dì èr míng.
Rán'ér, duonián lái, jiàoyù de zhòngdian cóng zongjiào zhuanxiàng shìsú wénhuà, cóng gu luóma jingdian dào zìji guójí de zuòjia suo chuàngzuò de zuòpin. Dúzhe zìji de yuyán zhong de wénzì chéngwéi wénxué kèchéng de zhuyào nèiróng. Zhège xiangfa shì xinshang zuòzhe de wénxué fenggé. Xuésheng kenéng tongguò mófang huò zhìshao xinshang yingguó xuézhe ma xiu·anuòdé suowèi de “yigèrén de wénhuà zhong zuì hao de sixiang huò xiezuò” lái xuéxí rúhé xie de gèng hao. Zài cháng chunténg fùgài de jiànzhú wù hé xuéshù jiè de tíngyuàn li, xuézhe sikao hé taolùnle tamen de wénhuà yíchan de xìjié. Ta de jiàzhíguan jízhong yú lijie hé baocún chuántong de wénxué wénben, zhuyào shi cóng gudian gudài. Duì yuánwén de zhongshí shì zhè zhong jiangxuéjin de lixiang.
To identify sporadic ALS iPSC lines whose iMNs degenerated more rapidly than controls, we generated iPSCs from 6 sporadic ALS patients. We did not bias our selection of sporadic ALS samples based on genetic or clinical information. Whole-genome or -exome sequencing and repeat-primed PCR for the C9ORF72 locus showed that the 6 sporadic ALS patients did not contain rare variants in known ALS genes, nor a C9ORF72 repeat expansion (Supplemental Figure 1, A and B, and Supplemental Tables 1 and 2; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.127736DS1).
Wénlín de héxīn shǐmìng, rú gōngsī xīn zhāngchéng zhōng suǒ guīdìng, shì wèile bāngzhù rénmen xuéxí yǔyán, zhǔyào shì Hànyǔ hé Yīngyǔ; cùjìn bùtóng wénhuà jiān de jiàoyù, lǐjiě, xīnshǎng, hépíng, hézuò, tuánjié yǐjí duōyàngxìng; fāzhǎn yǔyán hé jiàoyù kēxué, jìshù hé jìqiǎo. Duìyú Wénlín Hànyǔ Xuéxí Ruǎnjiàn hé ABC xìliè cídiǎn dāngqián jí wèilái de yònghùmen, chúle tōngguò wéihù, kuòzhǎn, gǎishàn, xǔkě hé chūbǎn zhèxiē zuòpǐn hé qítā zuòpǐn lái zhīchí tāmen, Wénlín hái zhìlìyú wéihù zhèxiē zuòpǐn de wánzhěng, shēngyù hé yuánzé, bǐngchéng le tóngshí shǐyòng pīnyīn (pīnyīn wénzì) hé Hànzì (gèzhǒng xíngshì de Hànyǔ wénzì) de yuánzé miànxiàng Hànyǔ xuéxí, zhēng chuàng zhǔnquèxìng, xuéshùxìng, kěkàoxìng, gōngpíngxìng, xiàoyòng hé zhìliàng de zuìgāo biāozhǔn. Cǐwài, zuòwéi yī ge SPC zǔzhī, Yánjiūsuǒ huì chuánshòu wǒmen de zhuānjiā tuánduì shǐyòng de jìnéng, ràng hòurén yánxù wǒmen de shìyè.
Sep 2018: Our first IEEE TKDE paper has been accepted! The paper ‘‘Efficient and Distributed Generalized Canonical Correlations Analysis for Big Multiview Data’’ comes from a collaborative work with CMU (Prof. Christos Faloutsos and Prof. Tom Mitchell). Now the team members are spread across the U.S. and the world (OSU,UFL,CMU,UVA,UCR,IIS). Congratualations to all! The full paper will be uploaded soon.
Guānyú “Hàn-Yīng Yànyǔ Cídiǎn”: Xiàndài Yǔyán qīkān duì Luósēnnuò (Rohsenow) cídiǎn de píngjià shì “dàigěi rén yúkuài tǐyàn, yìyú shǐyòng ... wèi pǔtōng Yīngyǔ dúzhě kāiqǐ le Zhōngguó mínjiān zhìhuì de bǎokù ... duì rènhé jíbié de yǔyán xuéshēng hé rènhé xūyào jīngpì géyán de rén dōu shì jí jù xīyǐnlì qiě shífēn shòuyì de.” (89, 2005). Yóu Wénlín Yánjiūsuǒ (zhǐzhìbǎn de biānzhìzhě) kāifā de xīnbǎn ruǎnjiàn wèi xuéxí yànyǔ zhè yī bǎoguì zīyuán zēngtiān le xīn de wéidù. Ruǎnjiàn bǎnběn zhuān wèi pèihé Wénlín Hànyǔ Xuéxí Ruǎnjiàn 4.2 huò gèng gāo bǎnběn shǐyòng ér shèjì. Yōngyǒu Wénlín qiángdà de ABC diànzǐ cídiǎn de quánbù jíhé, yìwèizhe jiǎntǐ hé fántǐ Zhōngwén de dúzhě kěyǐ shíshí fǎngwèn gèzhǒnggèyàng de cítiáo yǐ tànqiú Luósēnnuò jiàoshòu jīngliáng fānyì de wēimiào zhī chù, bìng lǐjiě Zhōngwén yànyǔ gēng shēnkè de hányì.
Such a characteristic of we-media has further squeezed the price bidding space of the new media of Fujian press industry in the area of content in local journalists’ eyes. What is worse, the procedure of three-phase censorship that deviates from the logic of new media is not strictly followed during the course of converging news production. Delays in the course of censorship have not only impaired the market competitiveness of the new media in terms of content, but also considerably reduced the willingness of journalists to collaborate with the new media centre.

Wo 11 yuè 24 rì fei wang zhongguó, huale shí tian shíjian yu lián. Women jìhuà qù yuènán luxíng, dànshì yóuyú wo méiyou zhòng wén de wénjiàn, suoyi méiyou dédào jiejué. Women ye xiwàng qù tianjin yitian de luxíng, dàn méiyou. Women huale zhengzheng yigè shíjian zài beijing, zài nàli maile xin yanjìng, bìng pèile yìchi. Wo yigè rén huí dào míngnísudá zhou,12 yuè 5 rì língchén dàodá.
Zài 2009 nián de zuìhòu yigè yuè, wo kaishi chuàngjiàn yigè míng wèi “BillMcGaughey.Com” de wangzhàn, zuòwéi wo wangzhàn zuòpin de jiaodian. Wo cóng niánqing rén nàli huànle rongcháng de shougao. Gèrén gùshì jiang shì wo de zhuancháng. Rán'ér, liánxù bùduàn de bàoyuàn wo de zìsi. Zhè ji tian, women hen shao zài tóngyi zhang chuángshàng shuìjiào. Lián zài lìng yigè danwèi li you zìji de wòshì. Ta huáiyí wo kenéng huàn you àizibìng. Rán'ér, ta yuè lái yuè duo de hua zài zhongguó, yibiàn yisheng nénggòu jiancè bìngqíng, kàn kàn áizhèng shìfou yijing fanhuí.
In recent years, the Fujian press industry has stagnated due to frequent turnover of personnel. Given that the number of practicing journalists in this province is difficult to determine, the authors adopted the snowball sampling method by firstly contacting the persons-in-charge of the newsroom and requesting them thereafter to recruit journalists to answer the survey. Considering the particularity of the respondents’ profession, that is, having flexible working hours, two assistants were assigned to distribute the printed questionnaires before and after the plenary press conference or the newsroom convention from July to August 2015.
Protein quantity was measured by the BCA assay (Pierce) and the proteins were run in a 10% SDS gel at 4°C, and the proteins were transferred onto an Immobilon membrane (Millipore). The membrane was blocked with 5% milk in 0.1% PBS-T (Sigma-Aldrich), incubated with primary antibodies overnight at 4°C, washed 3 times with 0.1% PBS-T, and then incubated with horseradish peroxidase–conjugated (HRP-conjugated) secondary antibody (Santa Cruz Biotechnology). After 3 washes with 0.1% PBS-T, blots were visualized using an Amersham ECL Western Blotting Detection Kit (GE) or the SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) and developed on X-ray film (Genesee). The following primary antibodies were used: mouse anti-GAPDH (Santa Cruz Biotechnology, catalog sc-32233, 1:1000), mouse anti-NR1 (Novus, catalog NB300118, 1:1000), mouse anti–transferrin receptor (Thermo Fisher Scientific, catalog 136800, 1:1000), mouse anti-TUJ1 (Biolegend, catalog MMS-435P, 1:2000), rabbit anti-poly(GR) (Proteintech, catalog 23978-1-AP, 1:1000), anti-mouse–HRP (Cell Signaling Technology, catalog 7076S, 1:5000), anti-rabbit–HRP (Cell Signaling Technology, catalog 7074S, 1:5000), and anti-LC3 (VWR, catalog 101732-348, 1:1000).
From the viewpoint of local journalists, news from the Fujian press industry was originally highly homogenised and lacked competitiveness. After the three-phase censorship of new media was institutionalised, risks from converging news production declined, but the timely release of information was weakened and the opportunity for journalistic autonomy has decreased as well. When covering politically sensitive social events, the freedom of speech of the new media was equally limited relative to others within the press industry and was occasionally even more restricted than that of newspapers.
29 Wan, M., Sino-Japanese Relations: Interaction, Logic, and Transformation (Stanford: Stanford University Press, 2006); Reilly, J., “China's history activism and Sino-Japanese relations,” China: An International Journal, Vol. 4, No. 2 (2006), pp. 189–216; J.C. Weiss, “Powerful patriots: nationalism, diplomacy and the strategic logic of anti-foreign protest,” PhD thesis, University of California, San Diego, 2008.
Wénlín de héxīn shǐmìng, rú gōngsī xīn zhāngchéng zhōng suǒ guīdìng, shì wèile bāngzhù rénmen xuéxí yǔyán, zhǔyào shì Hànyǔ hé Yīngyǔ; cùjìn bùtóng wénhuà jiān de jiàoyù, lǐjiě, xīnshǎng, hépíng, hézuò, tuánjié yǐjí duōyàngxìng; fāzhǎn yǔyán hé jiàoyù kēxué, jìshù hé jìqiǎo. Duìyú Wénlín Hànyǔ Xuéxí Ruǎnjiàn hé ABC xìliè cídiǎn dāngqián jí wèilái de yònghùmen, chúle tōngguò wéihù, kuòzhǎn, gǎishàn, xǔkě hé chūbǎn zhèxiē zuòpǐn hé qítā zuòpǐn lái zhīchí tāmen, Wénlín hái zhìlìyú wéihù zhèxiē zuòpǐn de wánzhěng, shēngyù hé yuánzé, bǐngchéng le tóngshí shǐyòng pīnyīn (pīnyīn wénzì) hé Hànzì (gèzhǒng xíngshì de Hànyǔ wénzì) de yuánzé miànxiàng Hànyǔ xuéxí, zhēng chuàng zhǔnquèxìng, xuéshùxìng, kěkàoxìng, gōngpíngxìng, xiàoyòng hé zhìliàng de zuìgāo biāozhǔn. Cǐwài, zuòwéi yī ge SPC zǔzhī, Yánjiūsuǒ huì chuánshòu wǒmen de zhuānjiā tuánduì shǐyòng de jìnéng, ràng hòurén yánxù wǒmen de shìyè.

Wo 11 yuè 24 rì fei wang zhongguó, huale shí tian shíjian yu lián. Women jìhuà qù yuènán luxíng, dànshì yóuyú wo méiyou zhòng wén de wénjiàn, suoyi méiyou dédào jiejué. Women ye xiwàng qù tianjin yitian de luxíng, dàn méiyou. Women huale zhengzheng yigè shíjian zài beijing, zài nàli maile xin yanjìng, bìng pèile yìchi. Wo yigè rén huí dào míngnísudá zhou,12 yuè 5 rì língchén dàodá.

The survey (see Table 2) indicates that from the viewpoint of local journalists in Fujian, new media has generally expanded the sources of news and facilitated contact with news-related groups. However, new media has also raised considerably high requirements for journalists’ expertise and spawned fierce peer competitions. Moreover, the respondents relatively agreed with the influences of new media in terms of enhancing journalists’ knowledge about the audience, although they disagreed with the belief that new media has marginalised news gathering and editing. The respondents took relatively positive attitudes towards the overall influences of new media on news production.
Zài 2007 nián zhì 2009 nián qíjian, wo hen shao canyù xila de shenghuó. Genjù liánhé huì zài míngnísudá zhou de qíngkuàng, ta hé wo jiang jù zài yiqi. You yiduàn shíjian, ta hé wo zhùle ji gè jiequ, dànshì women méiyou jingcháng kàn dào duìfang. Zuìxiao de nu'ér ai li ka, huxi xìtong wèntí shidé chéngshì kongqì èhuà. Ránhòu,Sheila jiang ta de jiarén ban dàole wèiyú chéngshì dongnán bù jiaoqu de Cottage Grove, kongqì qingxin dì dìfang. Zhège shèqu zhuyào shi báisè de, zài mou xie qíngkuàng xià yeshì duì heirén de díyì. Zhè bi ta de háizi duo de daraole xila. Tamen hen róngyì zuò báisè de péngyou.
Dànshì, wo de xiezuò xing qù bìng méiyou tíngzhi. Zuòwéi kuàijìshi, wo shòudào qifa, yánjiu láogong tongjì shùzì, bìng jiànlì lìfa lái suoduan gongzuò shíjian. Zhè chéngwéi wo 1981 nián chuban de dì yi ben shu de zhutí. Wo hé wo de qizi zài báixióng hú maile yigè xiao fángzi. Wo yòng yigè pángbian de fáng jiàn zuòwéi wo de gèrén xuéxí. Shujià shàng kàojìn tianhuaban de jiàzi.

Lín dá duì zìji zài xuduo bùtóng qíngkuàng xià de pingé gan hen gan xìngqù. Ta kenéng shì yigè nu tóngzhì míni míngrén, yigè you bàofù de geshou, yigè heirén de gònghédang rén hé yigè qiángdà de nánrén de qíngfù. (Ta de zhuancháng zhèngzài péiyang yu NFL zhu jiàoliàn hé gao jíbié zhèngzhì jia de guanxì.) Ta zài bùshí 41 suì de shíhòu chéngwéi báigong shíxí sheng, dànshì dang ta tóusù zhongzú qíshìshí, ta bèi fàngkuanle zhège zhíwèi. Zài women de diànhuà zhong, ta shuo ta ganggang yù dàole kèla lún si·tuomasi. Tongguò ta, youxìng fangwènle yixie zhimíng rénshì dì gèrén shenghuó.
Hao xiaoxi shì, ta xiànzài zài fùguó yínháng youyi fèn gongzuò, yigè mei xiaoshí zhifù 15 meiyuán. Ta cóng wo nàli toule qián lái zhifù yínháng fèiyòng. Ta ye zhèngzài jìnrù wojia de lóu xià danwèi, bìng you jinjí yuánzhù zhifù yigè yuè de zujin hé sunhui yajin. Wo zuò chule yigè juédìng. Wo keyi xiàng meiguó yínháng bàogào qizhà xíngwéi bìng shouhuí zijin, ye kenéng jiang xila sòng dào jianyù, fouzé wo keyi jieshòu dàoqiè, xiwàng yiqiè shùnlì. Wo xuanzéle hòu zhe.
Yousan gè zhèyàng de zuhù. Wo zài tóngyi tian xiàwu xiàng tamen fachule quzhú tongzhi. Yi míng míng jiào jími de heisè zu kè, zhànjùle dàlóu de dì yi gongyù, xianrán shì yi míng dúfàn. Wo jingcháng kàn dào ta zhàn zài jiejiao, bàngqiú mào zhuan guòtóu lái, ràng gè zhong gè yàng de rén yi lèisì shengyì de jiaotán fangshì jìnxíng jiaoliú. Jími shì dàibu jìlù de rén zhi yi. Wo xiàle ta de danwèi, qiao mén, dì gei ta de quzhú tongzhi, jieshì shuo wo zhèngzài quzhú dàibu jìlù de rén.

Zài 1983 nián xiàtian de ji gè yuè li, wo hen míliàn ma sha. Ta 28 suì rán'ér, ta de zhíyè shengyá bi wo gèngjia xianjìn. Wo wéiyi de míngsheng jiùshì chubanle yi ben jiào duan de gongzuò shíjian de shu. Ma sha you yigè jiyú xiao qiyè hé shougongyè de jingjì de yuànjing. Women zài qúndao hú bian sànbù, zài diànying zhòng kànle “su fei de xuanzé”, qù diàoyú, zài hùnxie zhànqu canjiale yi chang xì, zài kan nóng héshàng dùguòle yigè xiàwu de shíjian, canjiale yicì qiánshui huódòng, yicì zài ta de míng ní abo lì si gongyù li zuò'ài. Wèntí shì wo hàipà shiqù ma sha. Ta ganjué dào zhè yidian, ránhòu la huílái. Women zài 6 yuè hé 7 yuè fàng huan, dàole 8 yuèdi, women de guanxì jiùshì tongguòle. Wo de yuehuì fangshì wo yeshì “zhìxí” de.
5 See, for example, Li, C.-c., Voices of China: The Interplay of Politics and Journalism (New York: Guilford Press, 1990); Lynch, D.C., After the Propaganda State: Media, Politics, and “Thought Work” in Reformed China (Stanford: Stanford University Press, 1999); Esarey, A., “Cornering the market: state strategies for controlling China's commercial media,” Asian Perspective, Vol. 29, No. 4 (2005), pp. 37–83; Zhao, Y., Communication in China: Political Economy, Power, and Conflict (Lanham: Rowman & Littlefield, 2008); Polumbaum, J. and Lei, X., China Ink: The Changing Face of Chinese Journalism (Lanham: Rowman & Littlefield, 2008).
Wúlùn shì zhongzú háishì qíta yuányin, wo de fùmu dou bù zànchéng wo yu xila de hunyin. Wo de muqin anpái wo de dìdì an dí hé 1960 nián bìyè de ai kè sai tè zhunbèi xuéxiào de yiqún xiàoyou yiqi qù zhongguó luxíng. ai kè sai tè bìyè sheng de luxíng tuán dangshí shì meiguó zhù huá dàshi de tian'anmén guangchang dà túsha. Wo bèi yaoqiú péibàn an dí, yi quèbao ta fúyòng ta di yàowù. Zhè cì luxíng yú 1996 nián 4 yuèdi kaishi, chíxùle san gè xingqí. Dang wo huí dào míng ní abo lì si shí, xila hé háizimen yiqi zoule, hái you dà píngmù diànshì hé qíta de cáiwù. Dang wo da diànhuà gei shànghai, méiyou rén huídá de shíhòu, wo you yigè zhèyàng de qíngkuàng.
On 25 April, the Chongzhen Emperor was said to have walked to Meishan, a small hill in present-day Jingshan Park. There, he either hanged himself on a tree,[19] or strangled himself with a sash. By some accounts, the emperor left a suicide note that said, "I die unable to face my ancestors in the underworld, dejected and ashamed. May the rebels dismember my corpse and slaughter my officials, but let them not despoil the imperial tombs nor harm a single one of our people."[20] According to a servant who discovered the emperor's body under a tree, however, the words tianzi (Son of Heaven) were the only written evidence left after his death.[21] The emperor was buried in the Ming tombs.
Retrovirus encoded by pMXs-mRFP-GFP-LC3 was transduced into converting iMN cultures 1 day after transduction with iMN reprogramming factors. On day 17, iMNs were treated with 50 nM bafilomycin and 10 nM inactive 3K3A-APC, 10 nM 3K3A-APC, 10 μM DMSO, or 10 μM rapamycin (Sigma-Aldrich, R8781-200UL) in combinations described in the text and figure legends for 24 hours before being fixed with 4% paraformaldehyde at 4°C for 1 hour. Motor neuron cultures were immunostained to detect HB9 levels using a mouse anti-HB9 antibody (Developmental Studies Hybridoma Bank, 1:10 dilution) at 4°C for 48 hours. Coverslips were imaged on a Zeiss LSM 800 confocal microscope. Quantification was performed using ImageJ.
Our data indicate that motor neurons derived from C9ORF72 and at least a subset of sporadic ALS patients share similar defects in autophagosome formation and glutamate receptor accumulation. Importantly, PAR1 activation by 3K3A-APC can reverse these C9ORF72 and sporadic ALS disease processes in vitro and in vivo (Figure 7). We draw several key conclusions from our findings.

Wo bù zài kaichele (xila de xiongdì mei gè yuè duhuì dài wo dào Chanhassen de Eckankar fúwù, makè·sitan lì dài wo qù luobótè·bèi lì de yanchàng huì.) Diànchí qùnián qiutian zài wo de che shàng siwáng, cóng wèi bèi tìhuàn ránhòu, jinnián zao xie shíhòu, méiyou wo de zhishì huò xila chushòu cheliàng. Zhiyào women qù mou gè dìfang, ta jiù kaiche. Ciwài, wo yijing tíngzhi xiezuò jianchá. Wo de zhipiào jìcúnqì zhong de tiáomù yijing tíngzhi. Xiànzài shouqu zujin de xila, zhàogù zhàngdan. Women de cáizhèng réngrán wéixian.
Zhè wèi nushì, céng rèn jiudiàn zong jingli, xiànrèn zhongguó dì yi jia guangdà gongsi jiudiàn yèwù bùmén. Ta 43 suì. Ta de shí ji suì de nu'ér zài yingguó xuéxíle yi nián. Suirán liánhé pìnqingle kouyì yuán, dàn women nénggòu zài yingyu huìhuà zhong xianghù lijie. Lìrú, ta gàosù wo zhongguó nurén hen róngyì shìyìng zhàngfu de qíngkuàng. “Rúguo jià gei yi zhi ji,(qizi) shì yi zhi ji. Rúguo jià gei yi zhi gou,(ta) jiùshì yi zhi gou,“ta shì zenme shuo de. Zhè ye gei wo liú xiàle shenkè de yìnxiàng, lián nénggòu cóng méiyou ruanmù luósi de pútáojiu píng zhong quchu ruanmù sai - zhè shì jiudiàn guanli shìyè zhongxué dào de yigè qiàomén. Ta sìhu shì yigè màoxian de, you cáihuá de nurén.

“Tilian zi canghai de xinglu wenhua: Zhang Yueran xiaoshuo ‘Shiniao’ de wenxue zaixian” 提炼自沧海的行旅文化:张悦然小说《誓鸟》的文学再现  (Traveling Culture Distilled from Oceans: Literary Representation in Zhang Yueran’s Novel Shiniao), presented at the 2011 International Conference on “Chinese Production of Knowledge and Asian Social Transformation,” in Zhanghua, organized by the Department of National Literature and Institute of Taiwan Literature, Zhanghua Normal University, 28-29 October 2011.
Protein quantity was measured by the BCA assay (Pierce) and the proteins were run in a 10% SDS gel at 4°C, and the proteins were transferred onto an Immobilon membrane (Millipore). The membrane was blocked with 5% milk in 0.1% PBS-T (Sigma-Aldrich), incubated with primary antibodies overnight at 4°C, washed 3 times with 0.1% PBS-T, and then incubated with horseradish peroxidase–conjugated (HRP-conjugated) secondary antibody (Santa Cruz Biotechnology). After 3 washes with 0.1% PBS-T, blots were visualized using an Amersham ECL Western Blotting Detection Kit (GE) or the SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) and developed on X-ray film (Genesee). The following primary antibodies were used: mouse anti-GAPDH (Santa Cruz Biotechnology, catalog sc-32233, 1:1000), mouse anti-NR1 (Novus, catalog NB300118, 1:1000), mouse anti–transferrin receptor (Thermo Fisher Scientific, catalog 136800, 1:1000), mouse anti-TUJ1 (Biolegend, catalog MMS-435P, 1:2000), rabbit anti-poly(GR) (Proteintech, catalog 23978-1-AP, 1:1000), anti-mouse–HRP (Cell Signaling Technology, catalog 7076S, 1:5000), anti-rabbit–HRP (Cell Signaling Technology, catalog 7074S, 1:5000), and anti-LC3 (VWR, catalog 101732-348, 1:1000).
However, the official belief of ‘sole responsibility for one’s own profits or losses’ that has been established since the marketisation of Chinese media industries made it impossible for the Fujian press industry to gain sustainable financial investments from the state. Hence, the present objective of media convergence has been to explore new pathways towards profitability. To date, only a few new media outlets in the Fujian press industry have garnered gains. This ‘adventitious feebleness’ in profitability is deemed by local journalists as a consequence of the ‘congenital deficiency’ in content as exacerbated by the censorship system.
In the past when our Weibo and WeChat accounts were not taken over (by the new media centre of Xiamen Daily)… Journalists running to the spot would make an instant call to our editors, saying how a piece was on WeChat… Nowadays, the journalists no longer have such enthusiasm… Anyway, whatever the achievement is, they are not ours. (Interviewee No. 8)
Suízhe 2008 nián de kaishi, liánxù zengzhang gèngjia bù'an. Ta zài jiali xiang yào luxíng hé gòuwù de shenghuó. Ta xiang zuò de yi jiàn shì shì zài NBA bisài zhòng kàn xiusidùn tàikong rén lánqiú míngxing yáomíng. Women zài 2008 nián zuò dàole zhè yidian. Lìng yi jiàn shì bèi tuichíle, jiùshì zài yigè tuoyi wu jùlèbù kàn luoti wu zhe. Liánxì cheng zhè shì “meiguó wénhuà” de yibùfèn.
C9-BAC mice (36) (>6 months old) were transcardially perfused with PBS and subsequently with 4% formaldehyde. Cryoprotection occurred in 20% sucrose. After snap freezing, tissue was sectioned by cryostat at 20-μm thickness and stained with the following primary antibodies: anti-poly(GP) (catalog 24494-1-AP, ProteinTech), anti-poly(GR) (catalog MABN778, Millipore), anti-poly(PR) (catalog 23979-1-AP, ProteinTech), anti-NR1 (catalog NB300118, Novus), and anti-MAP2 (catalog ab5392, Abcam). Antigen retrieval was achieved with Target Retrieval Solution (pH 9, Agilent, Dako) for the DPR staining. Images were collected using a Zeiss LSM800 confocal microscope. The scientist performing the DPR quantification was blinded to the genotype or treatment condition of the samples. Confocal microscopy images of DPR punctae were adjusted for brightness and contrast for optimal visualization of the DPR punctae; this occurred to the same extent for the vehicle- and APC-treated side of the hippocampus.
Zhèxie wangzhàn de tao lún yuqì chúncuì shì ganxìng de. Xìng shì wèile chansheng shenti de lèqù. Zài mou xie qíngkuàng xià, kenéng huì daozhì ài yu hunyin. Rán'ér, yi shengzhí wèi mùdì dì xìngbié sìhu chaochule cangbái. Wo zuòwéi yigè xúnqiú lèqù de nánrén, ránhòu, rúguo shíjian sìhu zhèngquè, wo huì gaibiàn zhutí, jieshì wo zhenzhèng xiang yào de shì yigè ying'ér. Wo bù jìdé zhège cèlüè shì youxiào de. Yóuyú wo chéngnuò hé lián jiéhun, suoyi wo zhèngzài chuli yi zhi shou bang zài bèihòu. Zuìzhong, wo zhongzhile wo de huìyuán zigé. Wo huì jìxù shou dào lièbiao zhong de fùnu de diànzi yóujiàn, dànshì bùnéng zài bù gengxin huìyuán zigé de qíngkuàng xià huífù.
Xila zàicì gòumai fángdìchan, dàn yòu shiqùle. Ta anpái chéngwéi fukàn hei si tíng si shì de háozhái de kanshou. Yicì huò liang cì, dang wo zoule, wo ba wo de gou dài dào nàli, yu xila de gou Rico yiqi zài caodì shàng wánshua. Xila de muqin dangshí ye zhù zài nàli. Xila xuyào cóng wo jiè qián lái zhifù zujin, chéngnuò ta muqin de zujin fèn'é jíjiang dàolái. Bùguò, zhè bi qián zhuan ér zài muqin de judòng zhiqián bèi pài wang kansàsi chéng de lìng yi wèi nu'ér. Wo bèi yíqìle. Zhurén hen kuài jiù gan zoule xila hé ta de jiarén. Ta méiyou wendìng de gongzuò.
Liù nián qián, wo faxiàn zìji chuyú jiéhun zhuàngtài, dàn xianrán wúfa yu wo xiànzài de qizi yiqi sheng háizi. Wo kaishi rènshì dào, zài wo de qíngkuàng xià, hunyin shì yigè zuzhi wo chéngwéi fùqin de jigòu, ér bùshì yigè cùjìn fùqin shenghuó de jigòu. Zhè bùshì duì hunyin yinggai shì shénme de waili? Wo yiwéi shì zhèyàng, suoyi wo ba shìqíng jiao geile wo zìji de shou. Wo kaishi xúnzhao jihuì chéngwéi hun wài de fùqin. Jianchí shèhuì gongyue duì wo lái shuo yìyì bù dà. Wo shengmìng zhòng shèng xià de shíjian youxiàn. Wo bùdé bù caiqu xíngdòng.
Bùjiu zhihòu, xila jiù zhìliáo kekayin yilài. Ta fùfa yicì, ránhòu jìnrù lìng yigè zhìliáo fang'àn. Ta zhù zài Anoka de yigè zhongtú de fángzi yiduàn shíjian. Dang wo fangwèn shí, women youshí zài shì zhongxin de kafei diàn wán qí. Xila jingcháng ting jidujiào guangbò de línggan, ràng ta zou shàng fùsu de dàolù. Ta jíshí ba ta de háizi dài huílái, chéngwéi yigè rènzhen de muqin.
The historical course of China’s media reform is coincidental with the intrinsic logic of the transformation in national political ideology from contradiction theory to economy-centric theory (Li and Hu, 2013). However, this situation does not mean that political determinism would suffice to explain the China’s media reform. The transformation towards media groups did not result in mere innovation in the size, structure and managerial ideal of the media industries but also the ‘self-consciousness’ of actively promoting economic gains and the tendency to transform capitalism, ownership and other concepts into the reasonable kernel of media reform (Li and Hu, 2013). These have transcended far beyond the scope of what ‘political correctness’ can explain.
Liang gè màoyì rénmín, féi ní ji rén hé alabó rén, kaifale zìmu wénzì xìtong, qíta rénmín caiyòng. Xi bó lái rén zài dà wèi wáng tongzhì qíjian shiyòngle cóng féi ní ji pài lái de yigè jùben, dàn hòulái gai yòngle jiyú ala mu yu de jùben. Xilà zìmu ye yuán zì féi ní ji rén, zài gongyuán qián yiqian nián de zaoqí huò zhongqí kaishi shiyòng. ala ma yu de shangrén ba tamen de zìmu biao dong miàn chuanguò bosi hé afùhàn dào yìndù beibù. Zhè zhong chuàngxin de yìyì zàiyú, suízhe yìyú huòqu, gèng duo de rén keyi shiyòng shumiàn yuyán. Dúzhe gongzhòng bèi chuàngzàole.

Zhè shì yigè lumang de judòng. Zhulì'an dì èr tian zaoshang jiù líkai mìxixibi zhou, suirán ta huì duanzàn huí dào míngnísudá zhou, dàn ta jìhuà wúxiàn qí de zài nàli zhù. Ciwài, ta shì yigè zhongshí guanxì zhong de nu tóngzhì. Wo méiyou àn ta yào yigè baobao. Dànshì, rúguo wèilái de qíngkuàng shì zhèngquè de, wo quèshí xiwàng ta liaojie wo de yuànwàng. Rán'ér, yigè zhòngyào de yinsù shì, wo xiang kàn kàn wo shìfou you yongqì dàsheng shuo chu wo de xiangfa. You mèilì de nurén yizhí dou dònghè wo. Zhulì'an qíguài de shì you xiyin lì.

Jan. 2019: Check out this new submission: ‘‘Block-Randomized Stochastic Proximal Gradient for Low-Rank Tensor Factorization’’. This work uses a combination of randomized block coordinate descent and stochastic proximal gradient to decompose large and dense tensors with constraints and regularizations. The complexity saving is quite surprising. The total number of MTTKRPs (which dominates the CPD complexity) needed for the proposed algorithm is very small (see BrasCPD and AdaCPD in the figure).


In surveying pharmacological agents that might be capable of promoting autophagosome formation in C9ORF72 ALS iMNs, we identified activated protein C as a possible candidate. Activated protein C is an endogenous blood serine protease with both anticoagulant and cytoprotective activities, that latter of which is mediated by the activation of PAR1 through non-canonical cleavage (19). Zhong and colleagues previously showed that activated protein C treatment can rescue neurodegeneration in SOD1G93A mice (20) and promote autophagosome formation in murine lung epithelial cells (21). However, it remained unclear if activated protein C could stimulate autophagosome formation in C9ORF72 ALS iMNs, rescue neurodegeneration in non-SOD1 forms of ALS, or rescue neurodegenerative processes in patient-derived motor neurons. To test these possibilities, we used 3K3A-APC, an engineered form of activated protein C in which 3 active-site lysine residues required for degrading factor Va and VIIIa are changed to alanine, resulting in a 90% reduction in anticoagulant activity but full retention of PAR1 activation ability (22). 3K3A-APC is well tolerated in mice under chronic dosing at levels that activate PAR1 within the central nervous system, and short-term peripheral administration is safe in monkeys (20, 23, 24). As a negative control for these studies, we used heat-inactivated 3K3A-APC.
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