3K3A-APC also rescued the survival of iMNs from all 6 sporadic ALS lines (Figure 5H and Supplemental Figure 6, A–F; 6 sporadic ALS patients). When we tested iMNs from separate iPSC clones of the sporadic patient lines, 3K3A-APC also significantly improved their survival, indicating that the rescue effects were not specific to iMNs derived from certain iPSC clones (Supplemental Figure 6, G–J; 3 sporadic ALS patients). Reinforcing the notion that activating autophagosome formation in ALS iMNs could increase survival, rapamycin treatment significantly improved the survival of iMNs from 4 of 5 sporadic ALS lines tested (Supplemental Figure 7, A–J; 6 sporadic ALS patients). In contrast, rapamycin slightly decreased the survival of control iMNs (Supplemental Figure 7, K and L). These results suggest that rescuing autophagosome formation in sporadic ALS iMNs can increase their survival.
Suízhe shumiàn zhishì shèntòu dào gongzhòng, ta weixiéle céngjing longduàn zhè zhong yìshù de guanliáo de tèquán dìwèi. Fójiàoduì yìndù póluómén shèng zhí de zhíjie tiaozhàn. Zhéxué jia rú zuo luósi tè hé bólatú shèxiang wángguó genjù zhèngyì héshàn de lixiang chóng sù. Lìng yi fangmiàn, kongzi huígùle shiqián zhongguó chuánqí de guówáng, tamen de tongzhì bèi jì zhù wèi tamen de shèhuì héxié.
As an alternative to the 7F iMN differentiation procedure used in Fig. 7 and S8, iMNs were differentiated from C9-ALS and control iPSC lines using a Dox-NIL system. (a, b) Survival of Dox-NIL iMNs with or without TMX2 reduction by shRNA transduction. Results from two control (a) or two C9-ALS (b) lines were averaged to create the survival curves shown. (c, d) The same iMN data depicted in (b) but separated by individual C9-ALS cell line to show the variability in responses. (e) Representative images of GFP+ (shRNA expressing) C9-ALS iMNs taken during the survival experiments. (f) RNA was harvested from iMN survival experiments at the endpoint and TMX2 mRNA levels were measured by qRT-PCR (normalized to GAPDH levels). For information on the patient lines used and numbers of iMNs analyzed for survival analysis, see Supplementary Table 4.
Liu Yu's mother Zhao Anzong (趙安宗) died on the same day that she gave birth to him, in 363. His father Liu Qiao (劉翹) later married Xiao Wenshou, although the date of the marriage is not known. She bore Liu Qiao two sons, Liu Daolian (劉道憐) and Liu Daogui (劉道規). Liu Yu was described to be highly deferential and respectful to her, as she was the only mother he knew. After he overthrew Huan Xuan, who seized the Jin Dynasty (265-420) throne in 403, he was created the Duke of Yuzhang, and she was honored as the Duchess Dowager of Yuzhang in 412. After he was created the Prince of Song in 419, she was honored as princess dowager. After he seized the Jin throne and established Liu Song in 420, she was honored as the empress dowager. By this point, Liu Yu was himself quite advanced in age (57), but he was described as so careful and so loving to her that he visited her every day without ceasing. After he died and was succeeded by his son Emperor Shao of Liu Song in 422, she was honored as grand empress dowager. She died in 423, and, pursuant to instructions that both she and Emperor Wu left, she was buried at the same site as Liu Qiao but not in the same tomb, as it would be difficult to conduct an imperial burial in the small tomb that Liu Qiao was buried.
To determine significant pathways, differentially expressed genes for inactive 3K3A-APC versus 3K3A-APC treatment at 3 days were uploaded and analyzed by using the IPA tool (Qiagen). We focused on “Top Canonical Pathways.” Only canonical pathways with –log(P value) greater than 1.5 were retained. P values were calculated by using Fisher’s exact test.
Suízhe 2008 nián de kaishi, liánxù zengzhang gèngjia bù'an. Ta zài jiali xiang yào luxíng hé gòuwù de shenghuó. Ta xiang zuò de yi jiàn shì shì zài NBA bisài zhòng kàn xiusidùn tàikong rén lánqiú míngxing yáomíng. Women zài 2008 nián zuò dàole zhè yidian. Lìng yi jiàn shì bèi tuichíle, jiùshì zài yigè tuoyi wu jùlèbù kàn luoti wu zhe. Liánxì cheng zhè shì “meiguó wénhuà” de yibùfèn.
The authors adopted a semi-structural interview and provided an outline for the journalists that mainly revolved around the following nine questions: (1) When did you start using new media in your daily work? (2) What is your reason for using new media? (3) What do you think is the role of new media in news production? (4) What measures have been adopted by the press group that you are currently working for in terms of media convergence? (5) Did such measures result in changes in your daily work? If yes, what are such changes? What can you say about these changes? (6) How do you interpret the effects of media convergence on journalists based on your personal experience? (7) What is your overall evaluation of the media convergence within the press group you are working for? (8) Are you aware of the measures of other press groups in terms of media convergence? What is your opinion on their measures? (9) What can you say about the viewpoint of the local press industry that media convergence is the way out for its current predicament? Specific to the interviewees of other categories, the questions varied in terms of how they are stated and their order of arrangement.
The authors adopted a semi-structural interview and provided an outline for the journalists that mainly revolved around the following nine questions: (1) When did you start using new media in your daily work? (2) What is your reason for using new media? (3) What do you think is the role of new media in news production? (4) What measures have been adopted by the press group that you are currently working for in terms of media convergence? (5) Did such measures result in changes in your daily work? If yes, what are such changes? What can you say about these changes? (6) How do you interpret the effects of media convergence on journalists based on your personal experience? (7) What is your overall evaluation of the media convergence within the press group you are working for? (8) Are you aware of the measures of other press groups in terms of media convergence? What is your opinion on their measures? (9) What can you say about the viewpoint of the local press industry that media convergence is the way out for its current predicament? Specific to the interviewees of other categories, the questions varied in terms of how they are stated and their order of arrangement.

Zhè zhong tísheng dào changhé de nénglì you yigè jingshén céngmiàn. Jiézòu, xiàng xilà zhéxué de lixiang, you yidìng de tiansheng de pinzhí. Zhishì dì xìngzhì kenéng, kenéng, bangzhù yourén shengchan ta de xuqiú. Zài yu dingjí yùndòngyuán de fangtán zhong, xinli xué jia yijing xuéhuì rèn shì nénggòu dài lái gaofeng biaoxiàn de mou xie jìshù. Lìrú, keshìhuà jìshù bangzhù yùndòngyuán jingshén shàng jízhong yú shoutóu de rènwù. Gao'erfu qiú shou jiékè·níkè láo si (Jack Nicklaus) shuo, ta zài chángshì shèmén zhiqián jingcháng huì zài ta tóunao zhong “kàn diànying”.
Yóuyú mei gè fúhào dàibiao yigè chúncuì de sixiang yuánsù, zhèxie fúhào biàn chéng yu shuo chu de cí xiangguan lián. Shèjí wùli duìxiàng de cíyu keyi yóu zài shìjué shàng lèisì yú duìxiàng de xiàntiáo tú biaoshì. Lìrú, yigè yuánquan kenéng dàibiao tàiyáng; huòzhe, yinwèi tàiyáng meitian shàngsheng yicì, ta ye kenéng biaodá “tian” de gàiniàn. Cí de chouxiàng yìyì de yinjié zài yuyin shàng yu biaoshì wùli duìxiàng de cí xiangtóng, kenéng shiyòng xiangtóng de shìjué fúhào. Zuìzhong shumiàn yuyán huòdéle zài yuyán zhong zhaodào de mei gè cí de fúhào. Zhè shì biaoyì wénzì: Mei gè fúhào dàibiao yigè xiangfa, huàn jù huàshuo, yigè cí.
Zài qízhong yigè changhé, wo gàosù xila, wo shì tú zhaodào yigè méiyou jiéhun de nurén, yào hé wo yiqi shenghuó. Ta de guanzi bèi shùfù hòu, ta wúlì. Rán'ér, dang women jiéhun de shíhòu, women yijing jiandan dì wéi ta zuòle yigè shoushù lái niuzhuan shuluanguan jiéza. Zì nà yilái, zhè xiàng jìshù jìnbùle. Xila zài hùliánwang shàng jìnxíng sousuo, zài fóluólidá zhou de kèlì er wò tè zhaodàole yigè dìfang, jiang yi 10,000 meiyuán jìnxíng nìzhuan chéngxù. Ta rènwéi, boshìdùn kexué gongsi de jiànkang baoxian kenéng huì zhifù bùfèn chéngben. Rúguo you xìngqù, wo jiang bùdé bù zhifù qíyú fèiyòng.
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